DSMZ 104b . PY + X MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase peptone
(BD BBL)
5.0 g 5 -
Meat peptone
(pepsin-digested)
5.0 g 5 -
Yeast extract 10.0 g 10 -
Salt solution 40.0 ml - -
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 0.002
L-Cysteine HCl x H2O 0.5 g 0.5 2.847
D-Glucose 5.0 g 5 27.753
Distilled water 960.0 ml - -
1 Dissolve ingredients (except cysteine and glucose) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add cysteine and adjust pH to 7.0, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add glucose or any other substrate from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 6.8 - 7.0, if necessary.
Solution occurs in 9 other media (first defined in Medium 104).
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.25 g 0.25 1.701
MgSO4 x 7 H2O 0.50 g 0.5 2.029
K2HPO4 1.00 g 1 5.741
KH2PO4 1.00 g 1 7.348
NaHCO3 10.00 g 10 119.038
NaCl 2.00 g 2 34.223
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8 - 7.0
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 123
Strains with modifications: 22

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
D-Glucose 5 27.753
Trypticase peptone 5 -
Meat peptone 5 -
L-Cysteine HCl x H2O 0.5 2.847
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Cultivation metadata from 123 strains BacDive

pH 6 - 8 25 strains
pH 4 - 6 19 strains
pH 8 - 10 16 strains
pH 2 - 4 1 strain
phyla 3 121 strains
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