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104b. PY + X MEDIUM

Culture medium recipe

Vol.
×

M 104b #

ml
Equipment needed: Autoclave Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(BD BBL)
5.0 g 5 -
2
(pepsin-digested)
5.0 g 5 -
3 10.0 g 10 -
4 Salt solution 40.0 ml - -
5
(0.1% w/v)
0.5 ml 5.0e-4 0.002
6 0.5 g 0.5 2.847
8 1000.0 ml 998.207 55408.8
13 , required for pH adjustment
(5% w/v)
x - -
Instructions:
  1. Dissolve ingredients (except cysteine and glucose) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add cysteine and adjust pH to 7.0, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add glucose or any other substrate from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 6.8 - 7.0, if necessary.
Solution occurs in 6 other media (first defined in medium 104 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.25 g 0.25 1.701
2 0.50 g 0.5 2.029
3 1.00 g 1 5.741
4 1.00 g 1 7.348
5 10.00 g 10 119.038
7 1000.00 ml 998.207 55408.8
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.9
Equipment needed: Autoclave, Hungate tubes

Associated strains

All strains for this medium: 121
Strains with modifications: 119
104 PYG MEDIUM (MODIFIED)
a ANAEROBACULUM MEDIUM
b PY + X MEDIUM
c PY + X MEDIUM (N2/CO2)
d MALEDIVIBACTER MEDIUM (BETAINE)

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 1038.14 57625.2
Yeast extract 10 -
Trypticase peptone 5 -
Peptone from meat 5 -
L-Cysteine HCl x H2O 0.5 2.847
NaHCO3 0.4 4.762
KH2PO4 0.04 0.294
K2HPO4 0.04 0.23
MgSO4 x 7 H2O 0.02 0.081
CaCl2 x 2 H2O 0.01 0.068
Na-resazurin 5.0e-4 0.002

Cultivation metadata from 121 strains BacDive

halophily not 11 strains
halophily slightly 6 strains
halophily moderate 3 strains
halophily extreme 1 strain
pH 6 - 8 27 strains
pH 4 - 6 24 strains
pH 8 - 10 22 strains
pH 2 - 4 3 strains
pH 10 - 14 1 strain
phyla 3 114 strains
nutrition chemoorganotroph 4 strains
oxygen anaerobe 96 strains
oxygen obligate anaerobe 4 strains
oxygen facultative anaerobe 2 strains
temperature mesophilic 90 strains
temperature thermophilic 26 strains
temperature psychrophilic 3 strains
temperature hyperthermophilic 1 strain

Similar media

104a. ANAEROBACULUM MEDIUM 69 %
104c. PY + X MEDIUM (N2/CO2) 66 %
712. PSEUDOBUTYRIVIBRIO MEDIUM 63 %
152. NICOTINIC ACID MEDIUM 60 %
104d. MALEDIVIBACTER MEDIUM (BETAINE) 58 %

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