Strain modifications for Medium 684

7 distinct modifications were found.

Replace Na2-fumarate with 3.2 g/l sucrose added after autoclaving from a sterile anoxic solution prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 7.5.

Modified strains

Annotated modifications

Na2-fumarate
7.5
3.2 g Sucrose

Adjust pH of complete medium to 6.8.

Modified strains

Anaerosporomusa subterranea DSM 29728

Annotated modifications

6.8

Replace Na2-fumarate with 2.00 ml/l methanol added after autoclaving from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere.

Modified strains

Methanomethylovorans thermophila DSM 17232

Annotated modifications

Na2-fumarate
2 ml Methanol

Replace Na2-fumarate with 2.30 g/l glycerol added after autoclaving from a sterile anoxic solution prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 7.5.

Modified strains

Ercella succinigenes DSM 27333

Annotated modifications

Na2-fumarate
7.5
2.3 g Glycerol

Replace Na2-fumarate with 2.50 g/l Na-pyruvate added after autoclaving from a sterile anoxic solution prepared under 100% N2 gas atmosphere.

Modified strains

Desulfofundulus thermobenzoicus subsp. thermosyntrophicus DSM 14055

Annotated modifications

Na2-fumarate
2.5 g Na-pyruvate

Replace Na2-fumarate with 5.00 g/l glucose. Sterilize glucose separately by filtration under 100% N2 gas atmosphere. Reduce amount of Na2CO3 to 1.00 g/l and adjust pH to 6.5 - 6.8.

Modified strains

Victivallis vadensis DSM 14823

Annotated modifications

6.5 - 6.8
Na2CO3
Na2-fumarate
5 g Glucose
1 g Na2CO3

Replace Na2-fumarate with glycerol 2.3 g/L; anaerobic

Modified strains

Trichococcus flocculiformis DSM 23957

Annotated modifications

Na2-fumarate
2.3 g Glycerol