Loading ...

684. SYNTROPHOBACTER (MPOB) MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.53 g 0.524 2.942
2 0.41 g 0.405 2.977
3 0.30 g 0.296 5.542
4 0.11 g 0.109 0.739
5 0.10 g 0.099 0.486
6 0.30 g 0.296 5.072
7 Trace element solution SL-10 1.00 ml - -
8 Selenite-tungstate solution 1.00 ml - -
9 0.20 g 0.198 -
10
(0.1% w/v)
0.50 ml 4.9e-4 0.002
11 , might need strain adjustment 1.50 g - -
12 Vitamin solution 10.00 ml - -
13 3.20 g 3.162 19.758
14 0.50 g 0.494 2.057
15 1000.00 ml 986.371 54751.8
16 , might need strain adjustment 2.50 g/l - -
17 , might need strain adjustment 5.00 g/l - -
18 , might need strain adjustment 2.00 ml/l - -
19 , might need strain adjustment 2.30 g/l - -
20 , might need strain adjustment 3.20 g/l - -
Instructions:
  1. Dissolve ingredients (except carbonate, vitamins, fumarate and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving complete the medium by adding vitamins, fumarate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Stock solutions of vitamins and fumarate are sterilized by filtration. Adjust pH of the complete medium to 7.0 - 7.2. After inoculation pressurize culture vials to 0.7 bar overpressure with sterile 80% N2 and 20% CO2 gas mixture.
  2. Note: A white precipitate forms in this medium after autoclaving, which has however no negative effect on growth.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 7
Strains with modifications: 7

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.198 55408.3
Na2-fumarate 3.162 19.758
Na2HPO4 x 2 H2O 0.524 2.942
Na2S x 9 H2O 0.494 2.057
KH2PO4 0.405 2.977
Show more

Cultivation metadata from 7 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 4 7 strains
oxygen anaerobe 7 strains
oxygen obligate anaerobe 1 strain
Show more

Similar media

505. FORMIVIBRIO MEDIUM 86 %
1086. DESULFATIRHABDIUM MEDIUM 83 %
708. DESULFORHABDUS AMNIGENUS MEDIUM 82 %
503e. THERMODESULFITIMONAS MEDIUM 82 %
1718. METHANOSPIRILLUM F.2.7.3 MEDIUM 82 %

Latest comments

No comments for this medium.