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DSMZ 385 . DESULFOBACTERIUM CATECHOLICUM MEDIUM

Culture medium recipe

Vol.
×

Main sol. 385 #

ml
Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 932 ml - -
Solution B 1 ml - -
Solution C 20 ml - -
Solution D 10 ml - -
Solution E 10 ml - -
Solution F 10 ml - -
Solution G 10 ml - -
1 Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min ), then distributed under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclaved. Solutions B and G are autoclaved separately under 100% N2 gas.
2 Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D, E and F are prepared under 100% N2 gas and sterilized by filtration. The pyrocatechol stock solution has to be prepared always freshly prior to use. To complete the medium appropriate amounts of the solutions B to G are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be at 6.9 - 7.1.
3 Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 10 - 20% inoculum.

Solution A #

932
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SO4 3.00 g 3.219 22.662
KH2PO4 0.20 g 0.215 1.577
NH4Cl 0.30 g 0.322 6.018
NaCl 7.00 g 7.511 128.52
MgCl2 x 6 H2O 1.30 g 1.395 6.861
KCl 0.50 g 0.536 7.196
CaCl2 x 2 H2O 0.15 g 0.161 1.095
Selenite-tungstate solution 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 -
Distilled water 930.00 ml - -

Solution B #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace element solution SL-10 1 ml - -

Solution C #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 1 g 50 471.752
Distilled water 20 ml - -

Solution D #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-benzoate 0.4 g 40 277.579
Distilled water 10.0 ml - -

Solution E #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Pyrocatechol 0.06 g 6 54.491
HCl
(1 N) 		0.06 ml - -
Distilled water 10.00 ml - -

Solution F #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Wolin's vitamin solution 10 ml - -

Solution G #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 0.4 g 40 166.54
Distilled water 10.0 ml - -

Selenite-tungstate solution #

1000
Solution occurs in 173 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 262 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Wolin's vitamin solution #

1000
Solution occurs in 351 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.9 - 7.1
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes

Gas composition

  • 20 % CO2
  • 80 % N2

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 7.049 120.625
Na2SO4 3.021 21.27
MgCl2 x 6 H2O 1.309 6.439
Na2CO3 1.007 9.502
KCl 0.504 6.754
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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