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Main sol. 385

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Internal Solution-ID: S769

Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 952 ml - -
Solution B 20 ml - -
Solution C 10 ml - -
Solution D 10 ml - -
Solution E 1 ml - -
Solution F 10 ml - -
1 Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclaved. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solutions C and F are autoclaved under 100% N2 gas. Solutions D and E are prepared under 100% N2 gas and sterilized by filtration. The pyrocatechol stock solution has to be prepared always freshly prior to use. To complete the medium appropriate amounts of the solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be at 6.9 - 7.1.
2 Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 10 - 20% inoculum.
385 DESULFOCASTAENA MEDIUM