Strain modifications for Medium 830
135 distinct modifications were found.
pH 8.5
Modified strains
Annotated modifications
| 8.5 |
(leave out phosphate and pyruvate. Dilute 1:10 and adjust pH to 4.5 - 5.0 with alginic acid (or MES)); fill tubes for anaerobes (Hungate) half with medium, close tightly. Do not shake.
1/10 diluted
Modified strains
10-fold diluted and supplemented with 0.05% glucose
1:10 diluted, pH 4.5, without pyruvate, without phosphate
1:2 diluted
Modified strains
1:5 diluted, with cysteine and pyruvate (0.2 g/l each); keep agar surface moist
20% R2A; 20-37°C, pH 6.0-8.0 optimum
20-fold diluted and supplied with 0.05% glucose or fructose, pH 5.0 - 5.8
Add 0.06% sodium pyruvate
add 0.1% methylammoniumchloride
Modified strains
Annotated modifications
| 0.1 % Methylammonium chloride |
Add 0.5 M Na2CO3, pH 10.0
Add 1% methanol; aerobe
Adjust pH to 10 by adding 200 ml 0,5M Na2CO3
Adjust pH to 10 by adding 200ml 0.5M sodium carbonate [Na2CO3]
Adjust pH to 5.0 - 5.6 with HCl. Solidify with 5g/l agar
adjust pH to 5.5
Modified strains
Annotated modifications
| 5.5 |
Adjust the pH of the medium to 6.0
Aerobic, rehydrate and grow lyophilized cells in liquid medium. Subsequent subculturing may be carried out on agar plates also.
Aerobic. Rehydrate and grow lyophilized cells from ampoules in liquid broth 830. Subsequent subculturing may be carried out in liquid medium or on agar plates.
Aerobic; reactivate freeze dried cells in liquid medium.
Aerobic; reactivate lyophilized cells in liquid medium 830, incubate for at least 10 days
At 20 °C or 28 °C; (this medium is recommended for the routine cultivation of the strain)
braucht pH 8.5, wächst nicht auf pH 7.2
braucht pH 8.5, wächst nicht auf pH 7.2 nach LN
cover agar with liquid medium 830
Modified strains
Cultivate in liquid medium, only thin colonies on agar plate
Dilute R2A medium 1:5 and add Na-pyruvate and cysteine-HCl x H2O, 2 g per L each
Dilute the medium 10 fold and adjust the pH to 4.5-5.0
Dilute the medium 10 fold and adjust the pH to 5.0-5.5
Dilute the medium 10 fold, add 0.02% glucose and adjust the pH to 5.0 - 5.5
Dilute the medium 1:10, adjust the pH to 5.5
Dilute the medium 20 fold and add 0.05% fructose, adjust pH 4.0 - 5.0
diluted 1:1
Modified strains
Diluted 1:5. Add Na-pyruvate and cysteine-HCl x H2O, 0,2 g per L each
Growth is better on solid media if the surface is covered with a layer of liquid medium
half concentrated and prepared with 75% seawater
Modified strains
Keep agar surface moist and incubation atmosphere humid
Keep agar surface moist during incubation. No visible growth in liquid media
Keep agar surface moist during the whole incubation period
Keep agar surface moist with a film of liquid medium
liquid
Modified strains
Annotated modifications
| Agar |
M.830plus 50mg/L Rifampicin
Modified strains
Microaerobic, growing at 2-4% oxygen in atmosphere
Only one passage beginning with mineral medium with hexane is possible
pH 5,5
Modified strains
Annotated modifications
| 5.5 |
PH 5.0
pH 5.5
Modified strains
Annotated modifications
| 5.5 |
PH 5.5, use gelrite instead of agar
PH 5.9
PH 7
PH 7.0
Modified strains
Annotated modifications
| 7.0 |
PH 7.0 - 8.0
PH 8.0
PH 9.0
Plus 0.1% Tween 80
Plus 1% methanol
Plus 1.0 g/l NaCl
Plus 10 mg/l MnSO4
Plus 10 mg/L MnSO4
Plus 10 mg/L MnSO4; pH 7.0
Plus 10 mg/L MnSO4; pH 8.5
Plus 10 µg/ml MnSO4
Plus 10% NaCl
Plus 2g/l methanol
Plus 3% NaCl plus 10 mg/L MnSO4; pH 7.0
Plus 30 g/l NaCl
Plus MnSO4
Plus MnSO4 (10 mg/l)
Plus MnSO4 (10mg/l)
Plus Na-sesquicarbonate solution (see medium 31); pH 9.0
Plus vitamin solution (2ml/L); see medium 141
Plus 0,1% Methylamine
plus 0.5 g/l mannitol
plus 30 g/l NaCl
Modified strains
Annotated modifications
| 30 g/l NaCl |
Preferably liquid medium
Reactivate freeze dried culture in medium 830 (liquid medium and agar plate) and incubate for up to 14 days
Reactive in M. 1712; aerobic
Rehydate and grow freeze dried cells from ampoule in liquid medium 830. Subsequent subcultivation may be carried out in liquid medium or on agar plates, or in medium 457 with styrol
Rehydrate and grow freeze dried cells from ampoule in liquid medium 830. Subsequent subsculturing may be carried out in medium 465 with TM or on agar plates, too.
Rehydrate and grow freeze-dried cells from ampoule in liquid medium 54 or 830 first. Subsequent subcultivation may be carried out in liquid medium or on agar plates.
Rehydrate and grow freeze-dried cells from ampoule in liquid medium 830 first. Subsequent subcultivation may be carried out in liquid medium or on agar plates and in medium 457 with 3-chlorosalicylate.
Rehydrate and grow freeze-dried cells from the ampoule in medium 830, subsequent subculturing may be carried out in medium 457 with carbon source or in medium 830 or 464.
Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates
Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 830. Subsequent subculturing may be carried out in liquid medium or on agar plates
Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth. Subsequent subculturing may be carried out in liquid medium or on agar plates
Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth. Subsequent subculturing may be carried out in liquid medium or on agar plates.
Rehydrate and grow lyophylisate in liquid medium 830. Subsequently, subculturing may be carried out in liquid medium or on agar plates, on medium 830, 464 or 465 as indicated
Subculturing is recommended to be carried out on agar plates because growth in liquid medium is weak
Use half strength R2A liquid medium or agar.
Modified strains
With 10 mg/L MnSO4, adjust to pH 9 with Na-sesquicarbonate
With 1mg/l riboflavine
With 5 g/l NaCl
With 5 ml vitamin solution, see e.g. medium 461
With NaCl (20g/l) and MgCl2x 6H2O (3g/l)
With or without 10 mg/l 2,4,6-trichlorophenol and 20 mg/l pentachlorophenol
Omit Agar. Adjust pH of final medium to 6.0.
1 drop of 10x Wolin Vitamine Solution (see medium 141) and 10 microliter trace element solution SL-10 (see medium 320) for better growth in liquid
pH 10, plus 2% NaCl
pH 5,3
Modified strains
Annotated modifications
| 5.3 |
Plus 0,1% Tween 80
Plus 100 µl Anaerobiersalze (salt solution of DSMZ Medium 104)