Strain modifications for Medium 830

134 distinct modifications were found.

pH 8.5

Modified strains

Annotated modifications

8.5

(dilute 1:5)

Modified strains

Rubellimicrobium mesophilum DSM 19309

(leave out phosphate and pyruvate. Dilute 1:10 and adjust pH to 4.5 - 5.0 with alginic acid (or MES)); fill tubes for anaerobes (Hungate) half with medium, close tightly. Do not shake.

Modified strains

Telmatobacter bradus DSM 23630

Annotated modifications

K2HPO4
4.5 - 5.0
Na-pyruvate

(one-fifth-strength)

Modified strains

Ectobacillus panaciterrae DSM 19096

1/10 diluted

Modified strains

10-fold diluted and supplemented with 0.05% glucose

Modified strains

Mucilaginibacter paludis DSM 18603

Annotated modifications

0.5 g/l Glucose

1:10 diluted

Modified strains

Mucilaginibacter gotjawali DSM 29289

1:10 diluted, pH 4.5, without pyruvate, without phosphate

Modified strains

Acidobacteriaceae sp. DSM 24297

Annotated modifications

Na-pyruvate
4.5
K2HPO4

1:2 diluted

Modified strains

1:5 diluted, with cysteine and pyruvate (0.2 g/l each); keep agar surface moist

Modified strains

Polynucleobacter cosmopolitanus DSM 21490

Annotated modifications

Na-pyruvate
0.2 g/l Na pyruvate
0.2 g/l Cysteine

20% R2A; 20-37°C, pH 6.0-8.0 optimum

Modified strains

Oleiharenicola alkalitolerans DSM 29249

Annotated modifications

6.0 - 8.0

20-fold diluted and supplied with 0.05% glucose or fructose, pH 5.0 - 5.8

Modified strains

Bryocella elongata DSM 22489

Annotated modifications

0.5 g/l Glucose (or fructose)
5.0 - 5.8

Add 0.06% sodium pyruvate

Modified strains

Altererythrobacter palmitatis DSM 104092
Altererythrobacter lauratis DSM 104093

Annotated modifications

0.6 g/l Sodium pyruvate

add 0.1% methylammoniumchloride

Modified strains

Annotated modifications

0.1 % Methylammonium chloride

Add 0.5 M Na2CO3, pH 10.0

Modified strains

Arsukibacterium ikkense DSM 17999

Annotated modifications

0.5 M Na2CO3
10.0

Add 1% methanol; aerobe

Modified strains

Methylorubrum podarium DSM 15083

Annotated modifications

10.0 g/l Methanol

Adjust pH to 10 by adding 200 ml 0,5M Na2CO3

Modified strains

Rhodonellum ikkaensis DSM 17997

Annotated modifications

10.0

Adjust pH to 10 by adding 200ml 0.5M sodium carbonate [Na2CO3]

Modified strains

Rhodonellum psychrophilum DSM 17998

Annotated modifications

10.0

Adjust pH to 5.0 - 5.6 with HCl. Solidify with 5g/l agar

Modified strains

Granulicella arctica DSM 23128
Granulicella sapmiensis DSM 23136
Granulicella mallensis DSM 23137
Granulicella tundricola DSM 23138

Annotated modifications

5.0 g/l Agar
5.0 - 5.6

adjust pH to 5.5

Modified strains

Annotated modifications

5.5

Adjust pH to 7.5

Modified strains

Deinococcus sp. DSM 21675

Annotated modifications

7.5

Adjust the pH of the medium to 6.0

Modified strains

Terriglobus saanensis DSM 23119

Annotated modifications

6.0

Aerobic, rehydrate and grow lyophilized cells in liquid medium. Subsequent subculturing may be carried out on agar plates also.

Modified strains

Acidovorax sp. DSM 14692

Aerobic. Rehydrate and grow lyophilized cells from ampoules in liquid broth 830. Subsequent subculturing may be carried out in liquid medium or on agar plates.

Modified strains

Aromatoleum toluvorans DSM 15124

Aerobic; reactivate freeze dried cells in liquid medium.

Modified strains

Thauera aromatica DSM 14805

Aerobic; reactivate lyophilized cells in liquid medium 830, incubate for at least 10 days

Modified strains

Azospira oryzae DSM 11199

At 20 °C or 28 °C; (this medium is recommended for the routine cultivation of the strain)

Modified strains

Limnohabitans planktonicus DSM 21594

At half concentration

Modified strains

Gynumella flava DSM 21636

braucht pH 8.5, wächst nicht auf pH 7.2

Modified strains

Annotated modifications

8.5

braucht pH 8.5, wächst nicht auf pH 7.2 nach LN

Modified strains

Fertoeibacter niger DSM 108317

Annotated modifications

8.5

cover agar with liquid medium 830

Modified strains

Cultivate in liquid medium, only thin colonies on agar plate

Modified strains

Dechloromonas denitrificans DSM 15892

Dilute medium 1:1 with distilled water

Modified strains

Solirubrobacter ginsenosidimutans DSM 21036

Dilute R2A medium 1:5 and add Na-pyruvate and cysteine-HCl x H2O, 2 g per L each

Modified strains

Polynucleobacter acidiphobus DSM 21994

Dilute the medium 10 fold and adjust the pH to 4.5-5.0

Modified strains

Granulicella rosea DSM 18704

Annotated modifications

4.5 - 5.0

Dilute the medium 10 fold and adjust the pH to 5.0-5.5

Modified strains

Terriglobus sp. DSM 18693

Annotated modifications

5.0 - 5.5

Dilute the medium 10 fold, add 0.02% glucose and adjust the pH to 5.0 - 5.5

Modified strains

Acidicapsa borealis DSM 23886

Annotated modifications

0.2 g/l Glucose
5.0 - 5.5

Dilute the medium 1:10, adjust the pH to 5.5

Modified strains

Bryobacter aggregatus DSM 18758

Annotated modifications

5.5

Dilute the medium 20 fold and add 0.05% fructose, adjust pH 4.0 - 5.0

Modified strains

Granulicella paludicola DSM 22464

Annotated modifications

0.5 g/l Fructose
4.0 - 5.0

diluted 1:1

Modified strains

Diluted 1:2

Modified strains

Sediminibacterium magnilacihabitans DSM 21712

Diluted 1:5. Add Na-pyruvate and cysteine-HCl x H2O, 0,2 g per L each

Modified strains

Polynucleobacter difficilis DSM 22349

Growth is better on solid media if the surface is covered with a layer of liquid medium

Modified strains

Flavobacterium columnare DSM 18644

half concentrated and prepared with 75% seawater

Modified strains

half strengh

Modified strains

Nocardioides caricicola DSM 22177

Half strength substrate concentration

Modified strains

Ramlibacter ginsenosidimutans DSM 23480

Keep agar surface moist and incubation atmosphere humid

Modified strains

Limnohabitans australis DSM 21646

Keep agar surface moist during incubation. No visible growth in liquid media

Modified strains

Sphingomonas difficilis DSM 27573

Keep agar surface moist during the whole incubation period

Modified strains

Verminephrobacter aporrectodeae subsp. tuberculatae DSM 21895

Keep agar surface moist with a film of liquid medium

Modified strains

Polynucleobacter asymbioticus DSM 18221

liquid

Modified strains

Annotated modifications

Agar

Liquid media require heavy inoculum

Modified strains

Caenimonas terrae DSM 27942

Microaerobic (candle jar)

Modified strains

Pseudoxanthomonas spadix DSM 18855

Microaerobic, growing at 2-4% oxygen in atmosphere

Modified strains

Stenoxybacter acetivorans DSM 19021

Annotated modifications

3% O2

One-tenth diluted

Modified strains

Nocardioides baekrokdamisoli DSM 100725

One-tenth-strength

Modified strains

Capsulimonas corticalis DSM 105890

Only 20 mg/L of medium 830

Modified strains

Ramlibacter aquaticus DSM 110366

Only 25% strenth of the origianl medium 830

Modified strains

Dinghuibacter silviterrae DSM 100059

Only one passage beginning with mineral medium with hexane is possible

Modified strains

Alkanibacter difficilis DSM 14804

pH 5,5

Modified strains

Annotated modifications

5.5

PH 5.0

Modified strains

Actinocrinis puniceicyclus DSM 45618
Paraburkholderia eburnea DSM 29414

Annotated modifications

5.0

pH 5.5

Modified strains

Annotated modifications

5.5

PH 5.5, use gelrite instead of agar

Modified strains

Granulicella cerasi DSM 23641

Annotated modifications

5.5
Agar
15 g Gelrite

PH 5.9

Modified strains

Kyrpidia spormannii DSM 109470
Kyrpidia spormannii DSM 109471

Annotated modifications

5.9

PH 6.0

Modified strains

Caulobacter sp. DSM 26776

Annotated modifications

6.0

PH 6.0 - 8.0

Modified strains

Daejeonella lutea DSM 22385

Annotated modifications

6.0 - 8.0

PH 6.2

Modified strains

Flavobacterium reichenbachii DSM 21791

Annotated modifications

6.2

PH 6.5 - 8.0

Modified strains

Pedobacter sp. DSM 22386

Annotated modifications

6.5 - 8.0

PH 6.6

Modified strains

Kyrpidia spormannii DSM 106492

Annotated modifications

6.6

PH 7

Modified strains

Flavobacterium rivuli DSM 21788
Herbaspirillum sp. DSM 21937
Herbaspirillum sp. DSM 21938
Armatimonas rosea DSM 23562

Annotated modifications

7.0

PH 7.0

Modified strains

Arthrobacter alpinus DSM 22274
Agromyces bauzanensis DSM 22275
Pedobacter sp. DSM 22383
Pedobacter sp. DSM 22384
Parapedobacter luteus DSM 22899
Parapedobacter composti DSM 22900
Flavobacterium sp. DSM 23227
Arthrobacter cryoconiti DSM 23324
Caulobacter sp. DSM 23450
Labrenzia sp. DSM 23456
Herbaspirillum sp. DSM 23459
Nocardioides sp. DSM 23467
Oryzihumus sp. DSM 23468

Annotated modifications

7.0

PH 7.0 - 8.0

Modified strains

Paenibacillus sacheonensis DSM 23054

Annotated modifications

7.0 - 8.0

PH 7.2

Modified strains

Chelatococcus sp. DSM 21697

Annotated modifications

7.2

PH 7.3

Modified strains

Salinibacterium sp. DSM 22513

Annotated modifications

7.3

PH 8.0

Modified strains

Cecembia rubra DSM 28057
Rufibacter quisquiliarum DSM 29854

Annotated modifications

8.0

PH 8.5

Modified strains

Arundinibacter roseus DSM 106737

Annotated modifications

8.5

PH 9.0

Modified strains

Litchfieldia alkalitelluris DSM 16976
Nesterenkonia pannonica DSM 29786
Anaerobacillus alkaliphilus DSM 29790
Anaerobacillus alkaliphilus DSM 29794

Annotated modifications

9.0

PH: 9.5

Modified strains

Alkalilactibacillus ikkensis DSM 19937

Annotated modifications

9.5

PH=9.0

Modified strains

Nitrincola schmidtii DSM 100788

Annotated modifications

9.0

Plus 0.1% Tween 80

Modified strains

Paenibacillus pocheonensis DSM 23906

Annotated modifications

1.0 g/l Tween 80

Plus 1% methanol

Modified strains

Oharaeibacter diazotrophicus DSM 102969

Annotated modifications

10.0 g/l Methanol

Plus 1.0 g/l NaCl

Modified strains

Ohtaekwangia koreensis DSM 25262

Annotated modifications

1.0 g/l NaCl

Plus 10 mg/l MnSO4

Modified strains

Paenibacillus tianmuensis DSM 22342

Annotated modifications

10 mg/l MnSO4

Plus 10 mg/L MnSO4

Modified strains

Paenibacillus prosopidis DSM 22405

Annotated modifications

10 mg/L MnSO4

Plus 10 mg/L MnSO4; pH 7.0

Modified strains

Paenibacillus sp. DSM 23489
Paenibacillus sediminis DSM 23491

Annotated modifications

10 mg/L MnSO4
7.0

Plus 10 mg/L MnSO4; pH 8.5

Modified strains

Jeotgalibacillus soli DSM 23228

Annotated modifications

10 mg/L MnSO4
8.5

Plus 10 µg/ml MnSO4

Modified strains

Brevibacillus panacihumi DSM 22869

Annotated modifications

10 mg/l MnSO4

Plus 10% NaCl

Modified strains

Halomonas gomseomensis DSM 18042
Halomonas janggokensis DSM 18043
Salinicola salarius DSM 18044
Halomonas denitrificans DSM 18045
Nocardiopsis tangguensis DSM 45020
Nocardiopsis chakaensis DSM 45021

Annotated modifications

100.0 g/l NaCl

Plus 2g/l methanol

Modified strains

Hyphomicrobium sp. DSM 18203

Annotated modifications

2.0 g/l Methanol

Plus 3% NaCl plus 10 mg/L MnSO4; pH 7.0

Modified strains

Bacillus sp. DSM 23490

Annotated modifications

10 mg/L MnSO4
30.0 g/l NaCl
7.0

Plus 30 g/l NaCl

Modified strains

Wenyingzhuangia marina DSM 100572
Photobacterium galatheae DSM 100496

Annotated modifications

30.0 g/l NaCl

Plus MnSO4

Modified strains

Paenibacillus harenae DSM 16969
Neobacillus pocheonensis DSM 18135
Paenibacillus soli DSM 21316
Brevibacillus ginsengisoli DSM 21346
Paenibacillus ginsengihumi DSM 21568

Plus MnSO4 (10 mg/l)

Modified strains

Peribacillus kribbensis DSM 17871

Annotated modifications

10 mg/l MnSO4

Plus MnSO4 (10mg/l)

Modified strains

Paenibacillus gansuensis DSM 16968

Annotated modifications

10 mg/l MnSO4

Plus Na-sesquicarbonate solution (see medium 31); pH 9.0

Modified strains

Halalkalibacter akibai DSM 21942

Annotated modifications

9.0

Plus Tween 80

Modified strains

Georgenia daeguensis DSM 25321

Plus vitamin solution (2ml/L); see medium 141

Modified strains

Deinococcus antarcticus DSM 27864

Annotated modifications

+ 2 ml Wolin's vitamin solution

Plus 0,1% Methylamine

Modified strains

Novimethylophilus kurashikiensis DSM 105494

Annotated modifications

0.1 % Methylamine

plus 0.5 g/l mannitol

Modified strains

Phycicoccus sp. DSM 26743

Annotated modifications

0.5 g/l Mannitol

plus 30 g/l NaCl

Modified strains

Annotated modifications

30 g/l NaCl

Preferably liquid medium

Modified strains

Verminephrobacter eiseniae DSM 19249

Preferably liquid medium

Modified strains

Verminephrobacter eiseniae DSM 19250
Sphingomonas ginsengisoli DSM 18094

Reactivate freeze dried culture in medium 830 (liquid medium and agar plate) and incubate for up to 14 days

Modified strains

Chelativorans multitrophicus DSM 6780

Reactivate in M. 1712; aerobic

Modified strains

Aromatoleum petrolei DSM 19019

Reactive in M. 1712; aerob

Modified strains

Aromatoleum toluolicum DSM 19020

Reactive in M. 1712; aerobic

Modified strains

Aromatoleum aromaticum DSM 19016
Aromatoleum bremense DSM 19017
Aromatoleum aromaticum DSM 19018

Rehydate and grow freeze dried cells from ampoule in liquid medium 830. Subsequent subcultivation may be carried out in liquid medium or on agar plates, or in medium 457 with styrol

Modified strains

Xanthobacter sp. DSM 6696

Rehydrate and grow freeze dried cells from ampoule in liquid medium 830. Subsequent subsculturing may be carried out in medium 465 with TM or on agar plates, too.

Modified strains

Pseudomonas putida DSM 16845

Rehydrate and grow freeze-dried cells from ampoule in liquid medium 54 or 830 first. Subsequent subcultivation may be carried out in liquid medium or on agar plates.

Modified strains

Xanthomonas campestris DSM 2406

Rehydrate and grow freeze-dried cells from ampoule in liquid medium 830 first. Subsequent subcultivation may be carried out in liquid medium or on agar plates and in medium 457 with 3-chlorosalicylate.

Modified strains

Massilia dura DSM 17513

Rehydrate and grow freeze-dried cells from the ampoule in medium 830, subsequent subculturing may be carried out in medium 457 with carbon source or in medium 830 or 464.

Modified strains

Novosphingobium stygium DSM 12445

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates

Modified strains

Ramlibacter tataouinensis DSM 14655

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 830. Subsequent subculturing may be carried out in liquid medium or on agar plates

Modified strains

Hymenobacter arizonensis DSM 17870

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth. Subsequent subculturing may be carried out in liquid medium or on agar plates

Modified strains

Fulvimonas yonginensis DSM 28344

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth. Subsequent subculturing may be carried out in liquid medium or on agar plates.

Modified strains

Cellvibrio ostraviensis DSM 16616

Rehydrate and grow lyophylisate in liquid medium 830. Subsequently, subculturing may be carried out in liquid medium or on agar plates, on medium 830, 464 or 465 as indicated

Modified strains

Cupriavidus basilensis DSM 6708

Slow growth, poor growth in liquid media

Modified strains

Sphingomonas montana DSM 103337

Slowly growing, keep agar surface moist

Modified strains

Polynucleobacter rarus DSM 21648

Subculturing is recommended to be carried out on agar plates because growth in liquid medium is weak

Modified strains

Rhodanobacter soli DSM 25158

Twofold concentrated; incubation period 6-8 d

Modified strains

Oligoflexus tunisiensis DSM 103476

Use half strength R2A liquid medium or agar.

Modified strains

With 10 mg/L MnSO4, adjust to pH 9 with Na-sesquicarbonate

Modified strains

Halalkalibacter alkalisediminis DSM 21670

Annotated modifications

10 mg/L MnSO4
9.0

With 1mg/l riboflavine

Modified strains

Brevundimonas halotolerans DSM 24448

Annotated modifications

1.0 mg/l Riboflavine

With 5 g/l NaCl

Modified strains

Photorhabdus cinerea DSM 19717
Photorhabdus cinerea DSM 19724

Annotated modifications

5.0 g/l NaCl

With 5 ml vitamin solution, see e.g. medium 461

Modified strains

Thauera aminoaromatica DSM 25461

Annotated modifications

+ 5 ml Vitamin solution

With NaCl (20g/l) and MgCl2x 6H2O (3g/l)

Modified strains

Tessaracoccus profundi DSM 21240

Annotated modifications

20 g/l NaCl
3 g/l MgCl2 x 6 H2O

With or without 10 mg/l 2,4,6-trichlorophenol and 20 mg/l pentachlorophenol

Modified strains

Sphingomonas fennica DSM 13665

Annotated modifications

20 mg/l Pentachlorophenol
10 mg/l 2,4,6-Trichlorophenol

1 drop of 10x Wolin Vitamine Solution (see medium 141) and 10 microliter trace element solution SL-10 (see medium 320) for better growth in liquid

Modified strains

Nocardioides ginsengagri DSM 21362

Annotated modifications

+ 1 ml Modified Wolin's mineral solution
+ 0.01 ml Trace element solution SL-10

PH 10, plus 2% NaCl

Modified strains

Natronoglycomyces albus DSM 106290

Annotated modifications

20.0 g/l NaCl
10.0

pH 10, plus 2% NaCl

Modified strains

Annotated modifications

10.0
20 g/l NaCl

pH 5,3

Modified strains

Annotated modifications

5.3

pH 6.5

Modified strains

Thermogemmatispora carboxidivorans DSM 45816

Annotated modifications

6.5

Plus 0,1% Tween 80

Modified strains

Nocardioides daejeonensis DSM 25320

Annotated modifications

0.1 % Tween 80

Plus 100 µl Anaerobiersalze (salt solution of DSMZ Medium 104)

Modified strains

Modifications
pH 8.5 (dilute 1:5) (leave out phosphate and pyruvate. Dilute 1:10 and adjust pH to 4.5 - 5.0 with alginic acid (or MES)); fill tubes for anaerobes (Hungate) half with medium, close tightly. Do not shake. (one-fifth-strength) 1/10 diluted 10-fold diluted and supplemented with 0.05% glucose 1:10 diluted 1:10 diluted, pH 4.5, without pyruvate, without phosphate 1:2 diluted 1:5 diluted, with cysteine and pyruvate (0.2 g/l each); keep agar surface moist 20% R2A; 20-37°C, pH 6.0-8.0 optimum 20-fold diluted and supplied with 0.05% glucose or fructose, pH 5.0 - 5.8 Add 0.06% sodium pyruvate add 0.1% methylammoniumchloride Add 0.5 M Na2CO3, pH 10.0 Add 1% methanol; aerobe Adjust pH to 10 by adding 200 ml 0,5M Na2CO3 Adjust pH to 10 by adding 200ml 0.5M sodium carbonate [Na2CO3] Adjust pH to 5.0 - 5.6 with HCl. Solidify with 5g/l agar adjust pH to 5.5 Adjust pH to 7.5 Adjust the pH of the medium to 6.0 Aerobic, rehydrate and grow lyophilized cells in liquid medium. Subsequent subculturing may be carried out on agar plates also. Aerobic. Rehydrate and grow lyophilized cells from ampoules in liquid broth 830. Subsequent subculturing may be carried out in liquid medium or on agar plates. Aerobic; reactivate freeze dried cells in liquid medium. Aerobic; reactivate lyophilized cells in liquid medium 830, incubate for at least 10 days At 20 °C or 28 °C; (this medium is recommended for the routine cultivation of the strain) At half concentration braucht pH 8.5, wächst nicht auf pH 7.2 braucht pH 8.5, wächst nicht auf pH 7.2 nach LN cover agar with liquid medium 830 Cultivate in liquid medium, only thin colonies on agar plate Dilute medium 1:1 with distilled water Dilute R2A medium 1:5 and add Na-pyruvate and cysteine-HCl x H2O, 2 g per L each Dilute the medium 10 fold and adjust the pH to 4.5-5.0 Dilute the medium 10 fold and adjust the pH to 5.0-5.5 Dilute the medium 10 fold, add 0.02% glucose and adjust the pH to 5.0 - 5.5 Dilute the medium 1:10, adjust the pH to 5.5 Dilute the medium 20 fold and add 0.05% fructose, adjust pH 4.0 - 5.0 diluted 1:1 Diluted 1:2 Diluted 1:5. Add Na-pyruvate and cysteine-HCl x H2O, 0,2 g per L each Growth is better on solid media if the surface is covered with a layer of liquid medium half concentrated and prepared with 75% seawater half strengh Half strength substrate concentration Keep agar surface moist and incubation atmosphere humid Keep agar surface moist during incubation. No visible growth in liquid media Keep agar surface moist during the whole incubation period Keep agar surface moist with a film of liquid medium liquid Liquid media require heavy inoculum Microaerobic (candle jar) Microaerobic, growing at 2-4% oxygen in atmosphere One-tenth diluted One-tenth-strength Only 20 mg/L of medium 830 Only 25% strenth of the origianl medium 830 Only one passage beginning with mineral medium with hexane is possible pH 5,5 PH 5.0 pH 5.5 PH 5.5, use gelrite instead of agar PH 5.9 PH 6.0 PH 6.0 - 8.0 PH 6.2 PH 6.5 - 8.0 PH 6.6 PH 7 PH 7.0 PH 7.0 - 8.0 PH 7.2 PH 7.3 PH 8.0 PH 8.5 PH 9.0 PH: 9.5 PH=9.0 Plus 0.1% Tween 80 Plus 1% methanol Plus 1.0 g/l NaCl Plus 10 mg/l MnSO4 Plus 10 mg/L MnSO4 Plus 10 mg/L MnSO4; pH 7.0 Plus 10 mg/L MnSO4; pH 8.5 Plus 10 µg/ml MnSO4 Plus 10% NaCl Plus 2g/l methanol Plus 3% NaCl plus 10 mg/L MnSO4; pH 7.0 Plus 30 g/l NaCl Plus MnSO4 Plus MnSO4 (10 mg/l) Plus MnSO4 (10mg/l) Plus Na-sesquicarbonate solution (see medium 31); pH 9.0 Plus Tween 80 Plus vitamin solution (2ml/L); see medium 141 Plus 0,1% Methylamine plus 0.5 g/l mannitol plus 30 g/l NaCl Preferably liquid medium Preferably liquid medium Reactivate freeze dried culture in medium 830 (liquid medium and agar plate) and incubate for up to 14 days Reactivate in M. 1712; aerobic Reactive in M. 1712; aerob Reactive in M. 1712; aerobic Rehydate and grow freeze dried cells from ampoule in liquid medium 830. Subsequent subcultivation may be carried out in liquid medium or on agar plates, or in medium 457 with styrol Rehydrate and grow freeze dried cells from ampoule in liquid medium 830. Subsequent subsculturing may be carried out in medium 465 with TM or on agar plates, too. Rehydrate and grow freeze-dried cells from ampoule in liquid medium 54 or 830 first. Subsequent subcultivation may be carried out in liquid medium or on agar plates. Rehydrate and grow freeze-dried cells from ampoule in liquid medium 830 first. Subsequent subcultivation may be carried out in liquid medium or on agar plates and in medium 457 with 3-chlorosalicylate. Rehydrate and grow freeze-dried cells from the ampoule in medium 830, subsequent subculturing may be carried out in medium 457 with carbon source or in medium 830 or 464. Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 830. Subsequent subculturing may be carried out in liquid medium or on agar plates Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth. Subsequent subculturing may be carried out in liquid medium or on agar plates Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth. Subsequent subculturing may be carried out in liquid medium or on agar plates. Rehydrate and grow lyophylisate in liquid medium 830. Subsequently, subculturing may be carried out in liquid medium or on agar plates, on medium 830, 464 or 465 as indicated Slow growth, poor growth in liquid media Slowly growing, keep agar surface moist Subculturing is recommended to be carried out on agar plates because growth in liquid medium is weak Twofold concentrated; incubation period 6-8 d Use half strength R2A liquid medium or agar. With 10 mg/L MnSO4, adjust to pH 9 with Na-sesquicarbonate With 1mg/l riboflavine With 5 g/l NaCl With 5 ml vitamin solution, see e.g. medium 461 With NaCl (20g/l) and MgCl2x 6H2O (3g/l) With or without 10 mg/l 2,4,6-trichlorophenol and 20 mg/l pentachlorophenol 1 drop of 10x Wolin Vitamine Solution (see medium 141) and 10 microliter trace element solution SL-10 (see medium 320) for better growth in liquid PH 10, plus 2% NaCl pH 10, plus 2% NaCl pH 5,3 pH 6.5 Plus 0,1% Tween 80 Plus 100 µl Anaerobiersalze (salt solution of DSMZ Medium 104)