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830. R2A MEDIUM

Culture medium recipe

Vol.
×

M 830 #

ml
Solution occurs in 2 other media (first defined in medium 830 )
Equipment needed: Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.50 g 0.5 -
2
(Difco no. 3)
0.50 g 0.5 -
3 0.50 g 0.5 -
4 0.50 g 0.5 2.775
5 0.50 g 0.5 -
6 0.30 g 0.3 2.726
7 0.30 g 0.3 1.722
8 0.05 g 0.05 0.203
9 , adjust if required 15.00 g 15 -
10 1000.00 ml 998.207 55408.8
Instructions:
  1. Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave

Associated strains

All strains for this medium: 1548
Strains with modifications: 209
830 R2A MEDIUM
a MEDIUM R2A MODIFIED
b MEDIUM R2A FOR DENITRIFYING BACTERIA
c REACTIVATION WITH LIQUID MEDIUM 830
d R2A MEDIUM (MICROAEROBIC)

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Agar 15 -
Glucose 0.5 2.775
Yeast extract 0.5 -
Proteose peptone 0.5 -
Casamino acids 0.5 -
Starch 0.5 -
Na-pyruvate 0.3 2.726
K2HPO4 0.3 1.722
MgSO4 x 7 H2O 0.05 0.203

Cultivation metadata from 1548 strains BacDive

halophily not 405 strains
halophily slightly 193 strains
halophily moderate 95 strains
halophily extreme 5 strains
pH 6 - 8 487 strains
pH 4 - 6 394 strains
pH 8 - 10 384 strains
pH 10 - 14 112 strains
pH 2 - 4 40 strains
pH 0 - 2 1 strain
phyla 12 1497 strains
nutrition chemoorganotroph 28 strains
nutrition chemoorganoheterotroph 8 strains
nutrition heterotroph 5 strains
nutrition chemoheterotroph 2 strains
nutrition chemolithotroph 1 strain
nutrition chemolithoautotroph 1 strain
oxygen aerobe 544 strains
oxygen facultative anaerobe 45 strains
oxygen anaerobe 30 strains
oxygen obligate aerobe 19 strains
oxygen microaerophile 14 strains
oxygen facultative aerobe 11 strains
temperature mesophilic 1393 strains
temperature psychrophilic 161 strains
temperature thermophilic 49 strains
temperature hyperthermophilic 1 strain

Similar media

830c. REACTIVATION WITH LIQUID MEDIUM 830 100 %
919. LIMNOBACTER MEDIUM 100 %
1492. HALF STRENGTH R2A 99 %
1641. DOUBLE CONCENTRATED 830 + 0,06% SODIUM PYRUVATE 99 %
1578. 1/10 R2A MEDIUM 99 %

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