830d
830d .
R2A MEDIUM (MICROAEROBIC)
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 830d #
ml
Equipment needed: Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 0.50 | g | 0.5 | - | |
|
Proteose peptone
(BD DIFCO no. 3) |
0.50 | g | 0.5 | - | |
| Casamino acids | 0.50 | g | 0.5 | - | |
| Glucose | 0.50 | g | 0.5 | 2.775 | |
| Starch , soluble | 0.50 | g | 0.5 | - | |
| Na-pyruvate | 0.30 | g | 0.3 | 2.726 | |
| K2HPO4 | 0.30 | g | 0.3 | 1.722 | |
| MgSO4 x 7 H2O | 0.05 | g | 0.05 | 0.203 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients, then sparge solution with 80% N2 and 20% CO2 gas mixture for at least 30 - 45 min to remove dissolved oxygen and to saturate the solution with CO2. Dispense solution in vials suitable for anaerobic cultures (e.g. Balch-type tubes or serum vials) to 75% volume under air atmosphere. Close vials with butyl rubber septa to prevent free exchange of oxygen with the external atmosphere and autoclave. Adjust pH of the autoclaved medium to 5.0 - 5.5 using a sterile stock solution of sodium carbonate (5% w/v), prepared under 80% N2 and 20% CO2 gas mixture. | |||||
Metadata
| Last modified: | 07.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 5.0 - 5.5 |
| Equipment needed: | Autoclave, Gassing station |
| Edit in Medium builder | |
Gas composition
- 75 % N2
- 15 % CO2
- 10 % O2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Glucose | 0.5 | 2.775 |
| Starch | 0.5 | - |
| Casamino acids | 0.5 | - |
| Proteose peptone | 0.5 | - |
| Yeast extract | 0.5 | - |
| Show more | ||
