Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
|Compound||Amount||Unit||Conc. [g/L]||Conc. [mM]|
(BD DIFCO no. 3)
|Starch , soluble||0.50||g||0.5||-|
|MgSO4 x 7 H2O||0.05||g||0.05||0.203|
|1 Dissolve ingredients, then sparge solution with 80% N2 and 20% CO2 gas mixture for at least 30 - 45 min to remove dissolved oxygen and to saturate the solution with CO2. Dispense solution in vials suitable for anaerobic cultures (e.g. Balch-type tubes or serum vials) to 75% volume under air atmosphere. Close vials with butyl rubber septa to prevent free exchange of oxygen with the external atmosphere and autoclave. Adjust pH of the autoclaved medium to 5.0 - 5.5 using a sterile stock solution of sodium carbonate (5% w/v), prepared under 80% N2 and 20% CO2 gas mixture.|
|Medium type:||Complex medium|
|Final pH:||5.0 - 5.5|
|Equipment needed:||Autoclave, Gassing station|
|Edit in Medium builder|
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
|Compound||Conc. [g/L]||Conc. [mM]|