1492
1492 .
HALF STRENGTH R2A
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1492 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 0.250 | g | 0.25 | - | |
|
Proteose peptone
(Difco no. 3) |
0.250 | g | 0.25 | - | |
| Casamino acids | 0.250 | g | 0.25 | - | |
| Glucose | 0.250 | g | 0.25 | 1.388 | |
|
Starch
(soluble) |
0.250 | g | 0.25 | - | |
| Na-pyruvate | 0.250 | g | 0.25 | 2.272 | |
| K2HPO4 | 0.150 | g | 0.15 | 0.861 | |
| MgSO4 x 7 H2O | 0.025 | g | 0.025 | 0.101 | |
| Agar | 15.000 | g | 15 | 44.599 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Na-pyruvate | 0.25 | 2.272 |
| Glucose | 0.25 | 1.388 |
| Casamino acids | 0.25 | - |
| Starch | 0.25 | - |
| Show more | ||
