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Main sol. 1492

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Internal Solution-ID: S3060

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.250 g 0.25 -
Proteose peptone
(Difco no. 3) 		0.250 g 0.25 -
Casamino acids 0.250 g 0.25 -
Glucose 0.250 g 0.25 1.388
Starch
(soluble) 		0.250 g 0.25 -
Na-pyruvate 0.250 g 0.25 2.272
K2HPO4 0.150 g 0.15 0.861
MgSO4 x 7 H2O 0.025 g 0.025 0.101
Distilled water 1000.000 ml - -
1 Adjust pH to 7.2 with K2HPO4 or KH2PO4.
2 Add agar after adjusting the pH, then boil to dissolve the agar
Agar 15.000 g 15 -
3 Autoclave at 121 °C for 15 min.
1492 HALF STRENGTH R2A