Main sol. 503a
Internal Solution-ID: S1059
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.20 | g | 0.2 | 1.47 | |
| NH4Cl | 0.25 | g | 0.25 | 4.674 | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.4 | 1.968 | |
| KCl | 0.50 | g | 0.5 | 6.707 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.15 | 1.02 | |
|
Yeast extract
(OXOID) |
1.00 | g | 1 | - | |
|
Casamino acids
(BD Bacto) |
1.00 | g | 1 | - | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| Na2CO3 | 1.50 | g | 1.5 | 14.153 | |
| Seven vitamins solution | 1.00 | ml | - | - | |
| Na-DL-lactate | 5.00 | g | 5 | 44.619 | |
|
DL-Dithiothreitol
(DTT) |
3.00 | g | 3 | 19.443 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, vitamins, lactate and DTT), sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving complete the medium by adding vitamins, lactate and DTT from anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins are sterilized by filtration. Adjust pH of the complete medium to 7.0 - 7.2, if necessary. | |||||
Media with this solution
| 503a | SPOROMUSA TMAO3 MEDIUM |