MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 503f

Download this solution as

CSV JSON PDF

Internal Solution-ID: S1088

Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 952 ml - -
Solution B 25 ml - -
Solution C 1 ml - -
Solution D 10 ml - -
Solution E 10 ml - -
Solution F 10 ml - -
1 Dissolve compounds of solution A except sulfur and sparge it with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Distribute under the same gas atmosphere into anoxic Hungate-type tubes or serum vials containing the appropriate amount of sulfur and autoclave at 110°C for 20 min. Solution B is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D, E, and F are autoclaved under 100% N2 gas. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.2 - 7.4, if necessary.
503f AMINITHIOPHILUS MEDIUM