DSMZ 503f . J.5.4.2-T.3.5.2 MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Gassing station Hungate tubes Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 942 ml - -
Solution B 25 ml - -
Solution C 10 ml - -
Solution D 10 ml - -
Solution E 10 ml - -
Solution F 10 ml - -
1 Dissolve compounds of solution A except sulfur and sparge it with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic.
2 Distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials that contain the appropriate amount of sulfur and autoclave at 110°C for 20 min. Solution B is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution C is prepared under 100% N2 gas and sterilized by filtration.
3 Solutions D, E, and F are autoclaved under 100% N2 gas. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.2 - 7.4, if necessary.
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.20 g 0.215 1.577
NH4Cl 0.25 g 0.268 5.015
NaCl 5.00 g 5.365 91.8
MgCl2 x 6 H2O 0.40 g 0.429 2.111
KCl 0.50 g 0.536 7.196
CaCl2 x 2 H2O 0.15 g 0.161 1.095
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Sulfur
(powdered)
10.00 g 10.73 334.569
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 0.002
Distilled water 940.00 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 1.25 g 50 471.752
Distilled water 25.00 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Wolin's vitamin solution 10 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casamino acids 2 g 200 -
Distilled water 10 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.2 g 20 -
Distilled water 10.0 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 0.3 g 30 124.905
Distilled water 10.0 ml - -
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 174 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.08.22
Source: DSMZ
Medium type: Complex medium
Final pH: 7.2 - 7.4
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sulfur 10.03 312.757
NaCl 5.015 85.815
Casamino acids 2.006 -
Na2CO3 1.254 11.829
KCl 0.502 6.727
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