Main sol. 529
Internal Solution-ID: S1173
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Glucose | 10.0 | g | 10 | 55.506 | |
| K2HPO4 | 1.0 | g | 1 | 5.741 | |
| NH4NO3 | 2.0 | g | 2 | - | |
| MgSO4 x 7 H2O | 0.2 | g | 0.2 | 0.811 | |
| MnSO4 x 7 H2O | 5.0 | mg | 0.005 | 0.033 | |
| FeSO4 x 7 H2O | 5.0 | mg | 0.005 | 0.018 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
| Yeast extract | 3.0 | g | 3 | - | |
|
Polypeptone
(BBL) |
0.3 | g | 0.3 | - | |
| Agar | 15.0 | g | 15 | 44.599 | |
| Distilled water | 900.0 | ml | - | - | |
| Na-sesquicarbonate solution | 100.0 | ml | - | - | |
| 1 After sterilization add sterile 1 M Na-sesquicarbonate solution (1 ml in 10 ml) to achieve a pH of 9.7 (see medium 31). | |||||
Media with this solution
| 529 | AMPHIBACILLUS MEDIUM |