Main sol. 546
Internal Solution-ID: S1205
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Tryptone | 10 | g | 10 | 140.687 | |
| Yeast extract | 5 | g | 5 | - | |
| NaCl | 10 | g | 10 | 171.116 | |
| Glucose | 1 | g | 1 | 5.551 | |
| Distilled water | 990 | ml | - | - | |
|
CaCl2 x 2 H2O
(1 M) |
5 | ml | 0.735 | 5 | |
|
MgSO4 x 7 H2O
(1 M) |
5 | ml | 1.232 | 5 | |
| 1 Adjust pH to 7.4. | |||||
| 2 Sterilize at 121°C for 15 min. Cool to 50°C and add 5 ml 1 M CaCl2 x 2 H2O and 5 ml 1 M MgSO4 x 7 H2O as sterile solutions. | |||||
Media with this solution
| 546 | LC BROTH MEDIUM |