Main sol. 616
Internal Solution-ID: S1322
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2HPO4 x 2 H2O | 0.36 | g | 0.36 | 2.536 | |
| KCl | 0.08 | g | 0.08 | 1.073 | |
|
Yeast extract
(OXOID) |
5.00 | g | 5 | - | |
|
Tryptone
(BD Bacto) |
10.00 | g | 10 | 140.687 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| Na2CO3 | 5.30 | g | 5.3 | 50.006 | |
| D-Glucose | 3.00 | g | 3 | 16.652 | |
| L-Cysteine HCl x H2O | 0.20 | g | 0.2 | 1.139 | |
| Na2S x 9 H2O | 0.20 | g | 0.2 | 0.833 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, glucose, cysteine and sulfide) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonate, dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add glucose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and adjust pH to 10 by adding sterile anoxic 3 N NaOH. | |||||
Media with this solution
| 616 | ALKALITHERMOBACTER MEDIUM |