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Main sol. 619

Internal Solution-ID: S1327

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Methanol 10.0 ml 7.92 247.176
(NH4)2HPO4 3.0 g 3 22.717
NaCl 1.0 g 1 17.112
MgSO4 x 7 H2O 0.2 g 0.2 0.811
FeSO4 x 7 H2O 10.0 mg 0.01 0.036
MnSO4 x 1 H2O 5.0 mg 0.005 0.033
Vitamin mixture 5.0 ml - -
Agar 15.0 g 15 44.599
Tap water 1000.0 ml - -
1 Sterilize methanol by filtration, and add to other components sterilized by autoclaving.
619 HYPHOMICROBIUM METHYLOVORUM MEDIUM