619
619 .
HYPHOMICROBIUM METHYLOVORUM MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 619 #
ml
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Methanol | 10.0 | ml | 7.92 | 247.176 | |
| (NH4)2HPO4 | 3.0 | g | 3 | 22.717 | |
| NaCl | 1.0 | g | 1 | 17.112 | |
| MgSO4 x 7 H2O | 0.2 | g | 0.2 | 0.811 | |
| FeSO4 x 7 H2O | 10.0 | mg | 0.01 | 0.036 | |
| MnSO4 x 1 H2O | 5.0 | mg | 0.005 | 0.033 | |
| Vitamin mixture | 5.0 | ml | - | - | |
| Agar | 15.0 | g | 15 | 44.599 | |
| Tap water | 1000.0 | ml | - | - | |
| 1 Sterilize methanol by filtration, and add to other components sterilized by autoclaving. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 0.2 | mg | 2.0e-4 | 8.2e-4 |
| Calcium pantothenate | 40.0 | mg | 0.04 | 0.084 |
| Folic acid | 0.2 | mg | 2.0e-4 | 4.5e-4 |
| Inositol | 200.0 | mg | 0.2 | 1.11 |
| Niacin | 40.0 | mg | 0.04 | 0.325 |
| p-Aminobenzoic acid | 20.0 | mg | 0.02 | 0.146 |
| Pyridoxine hydrochloride | 40.0 | mg | 0.04 | 0.195 |
| Riboflavin | 40.0 | mg | 0.04 | 0.106 |
| Thiamine-HCl x 2 H2O | 20.0 | mg | 0.02 | 0.054 |
| Choline | 100.0 | mg | 0.1 | 0.96 |
| Vitamin B12 | 2.0 | µg | 2.0e-6 | 1.5e-6 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 15.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | n.d. |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Methanol | 7.92 | 247.176 |
| (NH4)2HPO4 | 3 | 22.717 |
| NaCl | 1 | 17.112 |
| MgSO4 x 7 H2O | 0.2 | 0.811 |
| Show more | ||
