Main sol. 655
Internal Solution-ID: S1386
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 1.00 | g | 1 | 5.741 | |
| MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
| CaCl2 x 2 H2O | 0.02 | g | 0.02 | 0.136 | |
| Na2S2O3 x 5 H2O | 0.20 | g | 0.2 | 0.806 | |
| Na-pyruvate | 2.20 | g | 2.2 | 19.992 | |
|
Yeast extract
(Difco) |
4.00 | g | 4 | - | |
| (NH4)2SO4 | 1.00 | g | 1 | 7.568 | |
| Trace element solution SL-6 | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 6.8. | |||||
| 2 Boil the medium for few minutes. Fill in tubes with rubber stoppers under nitrogen gas. Sterilize at 121°C for 15 min. | |||||
Media with this solution
| 655 | PYE MEDIUM |