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Main sol. 708

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Internal Solution-ID: S1458

Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 0.53 g 0.528 3.719
KH2PO4 0.41 g 0.408 3.001
NH4Cl 0.30 g 0.299 5.586
CaCl2 x 2 H2O 0.11 g 0.11 0.745
MgCl2 x 6 H2O 0.10 g 0.1 0.49
Na2SO4 2.80 g 2.789 19.634
NaCl 0.30 g 0.299 5.113
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
Na2CO3 1.50 g 1.494 14.096
Wolin's vitamin solution (10x) 1.00 ml - -
Seven vitamins solution 1.00 ml - -
Na-propionate 2.00 g 1.992 20.737
Na2S x 9 H2O 0.50 g 0.498 2.073
Distilled water 1000.00 ml - -
1 Dissolve ingredients except carbonate, vitamins, propionate and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins, propionate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins should be sterilized by filtration. The pH of the complete medium should be 7.2 - 7.6.
708 DESULFORHABDUS AMNIGENA MEDIUM