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Main sol. 728

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Internal Solution-ID: S1477

Equipment needed: Filter Hungate tubes Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 0.53 g 0.53 3.733
KH2PO4 0.41 g 0.41 3.013
NH4Cl 0.30 g 0.3 5.609
MgCl2 x 6 H2O 0.10 g 0.1 0.492
NaCl 0.30 g 0.3 5.133
Na2SO4 3.00 g 3 21.121
Na-acetate 1.64 g 1.64 19.992
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 -
Na2CO3 1.50 g 1.5 14.153
CaCl2 x 2 H2O 0.11 g 0.11 0.748
Seven vitamins solution 0.50 ml - -
Na2S x 9 H2O 0.50 g 0.5 2.082
Distilled water 1000.00 ml - -
1 Dissolve ingredients except carbonate, calcium chloride, vitamins and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add calcium chloride, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. The pH of the complete medium should be 7.5 - 7.7.
728 DESULFOBACCA MEDIUM