Main sol. 745
Internal Solution-ID: S1523
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KH2PO4 | 1.00 | g | 1 | 7.348 | |
(NH4)2SO4 | 1.00 | g | 1 | 7.568 | |
NaCl | 50.20 | g | 50.2 | 859.001 | |
MgCl2 x 6 H2O | 0.20 | g | 0.2 | 0.984 | |
CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.34 | |
Trace element solution SL-8 | 1.00 | ml | - | - | |
Na-DL-malate | 3.60 | g | 3.6 | 20.219 | |
Yeast extract | 1.00 | g | 1 | - | |
Na2S2O3 x 5 H2O | 0.50 | g | 0.5 | 2.015 | |
Distilled water | 1000.00 | ml | - | - | |
1 Boil the medium under a stream of nitrogen gas for few minutes and distribute in screw-capped bottles or tubes. Autoclave at 121°C for 15 minutes. |
745 | RHODOBIUM MEDIUM |