Main sol. 752
Internal Solution-ID: S1538
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 10 | g | 10 | - | |
| Na2-citrate | 3 | g | 3 | 11.625 | |
| KCl | 2 | g | 2 | 26.828 | |
| MgSO4 x 7 H2O | 1 | g | 1 | 4.057 | |
| NaCl | 100 | g | 100 | 1711.16 | |
| Na2CO3 | 3 | g | 3 | 28.305 | |
|
MnCl2 x 4 H2O
(0.36g/l) |
1 | ml | - | - | |
|
FeSO4 x 7 H2O
(50g/l) |
1 | ml | - | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 Liquid medium may be prepared by adding all components together. Agar (2%) medium should be prepared without Na2CO3 and sterilised by autoclaving. Add 20-30 ml of a sterile 10% Na2CO3 solution to the autoclaved medium containing agar so that the final pH is 9.0. | |||||
Media with this solution
| 752 | HALOMONAS PANTELLERIENSE |