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Main sol. 762

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Internal Solution-ID: S1552

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 5.0 g 5 27.753
MgCl2 x 6 H2O 0.2 g 0.2 0.984
KH2PO4 1.0 g 1 7.348
KNO3 2.0 g 2 19.782
Na2CO3 5.4 g 5.4 50.949
NaHCO3 4.2 g 4.2 49.996
Distilled water 1000.0 ml - -
1 Prepare the medium without Na2CO3 and NaHCO3 in 900 ml distilled water and autoclave. Prepare the Na2CO3 and NaHCO3 in 100 ml water, sterilise separately and add to the cooled medium. Final pH 9.5-10.0.
762 HALOMONAS DESIDERATA MEDIUM