Main sol. 784a
Internal Solution-ID: S1590
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.2 | g | 0.2 | 1.467 | |
| MgCl2 x 6 H2O | 0.1 | g | 0.1 | 0.491 | |
| NH4Cl | 1.0 | g | 0.998 | 18.658 | |
| KCl | 0.2 | g | 0.2 | 2.677 | |
| NaCl | 60.0 | g | 59.88 | 1024.64 | |
| Trace element solution SL-11 | 1.0 | ml | - | - | |
| Na-acetate | 0.2 | g | 0.2 | 2.433 | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 68.0 | g | 67.864 | 640.301 | |
| NaHCO3 | 38.0 | g | 37.924 | 451.44 | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| Na2S x 9 H2O | 1.0 | g | 0.998 | 4.155 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except carbonate, bicarbonate, vitamins and sulfide, then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve carbonates and sulfide while gassing the head space only. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add vitamins from an anoxic stock solution sterilized by filtration. Adjust pH of the complete medium to 9.0 - 9.5. | |||||
Media with this solution
| 784a | METHANOCALCULUS NATRONOPHILUS MEDIUM |