MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 788

Download this solution as

CSV JSON PDF

Internal Solution-ID: S1593

Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.41 g 0.409 3.004
Na2HPO4 x 2 H2O 0.52 g 0.518 3.652
NH4Cl 2.40 g 2.393 44.734
MgCl2 x 6 H2O 0.40 g 0.399 1.962
Yeast extract 0.20 g 0.199 -
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
CaCl2 x 2 H2O 0.20 g 0.199 1.356
Na2CO3 1.50 g 1.496 14.11
D-Glucose 5.00 g 4.985 27.67
Seven vitamins solution 1.00 ml - -
Na2S x 9 H2O 0.30 g 0.299 1.245
Distilled water 1000.00 ml - -
1 Dissolve ingredients except calcium chloride, carbonate, glucose, vitamins and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add calcium chloride, glucose, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins should be sterilized by filtration. Prior to use adjust pH of complete medium to 7.2 - 7.4, if necessary.
788 CALORAMATOR MEDIUM