Main sol. 815
Internal Solution-ID: S1654
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2SO4 | 3.0 | g | 3 | 21.121 | |
| CaCl2 x 2 H2O | 6.0 | g | 6 | 40.812 | |
| NaCl | 40.0 | g | 40 | 684.463 | |
| KCl | 2.0 | g | 2 | 26.828 | |
| NH4Cl | 0.3 | g | 0.3 | 5.609 | |
| KH2PO4 | 0.2 | g | 0.2 | 1.47 | |
| MgCl2 x 6 H2O | 8.0 | g | 8 | 39.35 | |
| SrCl2 x 6 H2O | 0.1 | g | 0.1 | 0.375 | |
| Trace element solution SL-11 | 1.0 | ml | - | - | |
| MOPS | 3.0 | g | 3 | 14.336 | |
| Yeast extract | 1.0 | g | 1 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
|
Iron powder
(or nails) |
100.0 | g | 100 | 1790.67 | |
| Na-L-lactate | 3.6 | g | 3.6 | - | |
| Na2CO3 | 0.5 | g | 0.5 | 4.718 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except iron, lactate and carbonate, adjust pH to 6.0 with NaOH and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials containing 1 - 2 g iron powder or an iron nail. Autoclave for 30 min at 105°C. Before use add lactate from a sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Final medium pH should be 7.2. | |||||
Media with this solution
| 815 | DESULFOHALOTOMACULUM MEDIUM |