Main sol. 842
Internal Solution-ID: S1724
Equipment needed: Anaerobic workbench
Hungate tubes
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 150.00 | g | 150 | 2566.74 | |
| MgCl2 x 6 H2O | 15.00 | g | 15 | 73.782 | |
| KCl | 3.00 | g | 3 | 40.241 | |
| NH4Cl | 0.50 | g | 0.5 | 9.348 | |
| KH2PO4 | 0.35 | g | 0.35 | 2.572 | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.34 | |
| Yeast extract | 0.10 | g | 0.1 | - | |
| Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
| NaHCO3 | 2.00 | g | 2 | 23.808 | |
| Glucose | 1.20 | g | 1.2 | 6.661 | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Trace element solution SL-12 B | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Vitamin solution 7 | 1.00 | ml | - | - | |
| Distilled water | 997.00 | ml | - | - | |
| 1 Final pH 7.2 - 7.4 | |||||
| 2 Prepare the medium without yeast extract, Na2S x 9 H2O, NaHCO3, glucose and vitamin solution (prepared as anaerobic, sterile stock solutions). Boil the medium and cool under anaerobic gas mixture of N2:CO2 (80:20). Dispense medium into Hungate tubes or serum bottles using the same gas mixture. Autoclave. Add the remaining components to the medium once it has cooled and check the final pH. | |||||
Media with this solution
| 842 | HALOANAEROBACTER SALINARIUS MEDIUM |