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Main sol. 858

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Internal Solution-ID: S1751

Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 9.000 ml - -
Solution B 0.500 ml - -
Solution C 0.025 ml - -
Solution D 0.050 ml - -
Solution E 0.025 ml - -
Solution F 0.500 ml - -
1 Prepare solution A, distribute approbiate amounts of cholesterol (e.g. 5 mg for 10 ml medium) and solution A in anaerobic tubes, and autoclave anaerobically under 80% N2 + 20% CO2. Solutions B and F are also autoclaved under these conditions. Solution C, D and E may be prepared aerobically. After cooling, add to 9 ml solution A: 500 µl sol. B, 500 µl sol. F, 25 µl (one drop) sol. C and E, each, and 50 µl sol. D under anaerobic conditions. Adjust pH to 7.0±0.2.
858 ANAEROBIC CHOLESTEROL MEDIUM