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DSMZ

DSMZ 858 . ANAEROBIC CHOLESTEROL MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 858 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 9.000 ml - -
Solution B 0.500 ml - -
Solution C 0.025 ml - -
Solution D 0.050 ml - -
Solution E 0.025 ml - -
Solution F 0.500 ml - -
1 Prepare solution A, distribute approbiate amounts of cholesterol (e.g. 5 mg for 10 ml medium) and solution A in anaerobic tubes, and autoclave anaerobically under 80% N2 + 20% CO2. Solutions B and F are also autoclaved under these conditions. Solution C, D and E may be prepared aerobically. After cooling, add to 9 ml solution A: 500 µl sol. B, 500 µl sol. F, 25 µl (one drop) sol. C and E, each, and 50 µl sol. D under anaerobic conditions. Adjust pH to 7.0±0.2.

Solution A #

200
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 0.10 g 0.5 2.029
NH4Cl 0.10 g 0.5 9.348
CaCl2 0.02 g 0.1 0.901
NaNO3 0.16 g 0.8 9.412
Distilled water 200.00 ml - -

Solution B #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1 g 10 73.484
Distilled water 100 ml - -

Solution C #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trace element solution SL-10 1 ml - -
1 Trace elements SL-10 (see medium 320)

Solution D #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin solution 1 ml - -

Solution E #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Selenite-tungstate solution 1 ml - -

Solution F #

100
Equipment needed: Anaerobic workbench Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 8.4 g 84 999.917
Distilled water 100.0 ml - -

Vitamin solution #

1000
Solution occurs in 15 other media (first defined in Medium 461).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 50 mg 0.05 0.037
Pantothenic acid 50 mg 0.05 0.228
Riboflavin 50 mg 0.05 0.133
Pyridoxamine hydrochloride 10 mg 0.01 0.049
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Nicotinic acid 25 mg 0.025 0.203
Nicotine amide 25 mg 0.025 0.205
alpha-lipoic acid 50 mg 0.05 0.242
p-Aminobenzoic acid 50 mg 0.05 0.365
Thiamine-HCl x 2 H2O 50 mg 0.05 0.134
Distilled water 1000 ml - -
1 Stir for some hours, filter sterilize the solution.

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite-tungstate solution #

1000
Solution occurs in 179 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Metadata

Last modified: 24.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8 - 7.2
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.2 49.996
NaNO3 0.72 8.471
KH2PO4 0.5 3.674
NH4Cl 0.45 8.413
MgSO4 x 7 H2O 0.45 1.826
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Cultivation metadata from 2 strains BacDive

phyla 2 2 strains
oxygen anaerobe 2 strains
oxygen aerobe 1 strain
temperature mesophilic 2 strains