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858. ANAEROBIC CHOLESTEROL MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Anaerobic workbench
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 9.0 ml - -
2 Solution B 0.5 ml - -
3 Solution C 25.0 μl - -
4 Solution D 50.0 μl - -
5 Solution E 25.0 μl - -
6 Solution F 0.5 ml - -
Instructions:
  1. Prepare solution A, distribute approbiate amounts of cholesterol (e.g. 5 mg for 10 ml medium) and solution A in anaerobic tubes, and autoclave anaerobically under 80% N2 + 20% CO2. Solutions B and F are also autoclaved under these conditions. Solution C, D and E may be prepared aerobically. After cooling, add to 9 ml solution A: 500 μl sol. B, 500 μl sol. F, 25 μl (one drop) sol. C and E, each, and 50 μl sol. D under anaerobic conditions. Adjust pH to 7.0±0.2.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.10 g 0.5 2.029
2 0.10 g 0.5 9.347
3 0.02 g 0.1 0.901
4 0.16 g 0.8 9.412
5 200.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1 g 10 73.484
2 100 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace element solution SL-10 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Vitamin solution 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Selenite-tungstate solution 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 8.4 g 84 999.921
2 100.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.
Solution occurs in 17 other media (first defined in medium 1116 )
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 50 mg 0.05 0.032
2 50 mg 0.05 0.228
3 50 mg 0.05 0.133
4 10 mg 0.01 0.049
5 20 mg 0.02 0.082
6 20 mg 0.02 0.045
7 25 mg 0.025 0.203
8 25 mg 0.025 0.205
9 50 mg 0.05 0.242
10 50 mg 0.05 0.365
11 50 mg 0.05 0.134
12 1000 ml 998.207 55408.8
Instructions:
  1. Stir for some hours, filter sterilize the solution.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Anaerobic workbench, Autoclave, Filter

Associated strains

All strains for this medium: 2
Strains with modifications: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 78.252 4343.66
HCl 0.057 1.553
NaHCO3 0.03 0.357
FeCl2 x 4 H2O 0.027 0.135
NaOH 0.009 0.223
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Cultivation metadata from 2 strains BacDive

phyla 2 2 strains
oxygen anaerobe 2 strains
oxygen aerobe 1 strain
temperature mesophilic 2 strains

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