Main sol. 864
Internal Solution-ID: S1785
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 29.00 | g | 28.856 | 493.767 | |
(NH4)2SO4 | 1.00 | g | 0.995 | 7.53 | |
MgSO4 x 7 H2O | 1.50 | g | 1.493 | 6.056 | |
CaCl2 x 2 H2O | 0.42 | g | 0.418 | 2.843 | |
KCl | 0.70 | g | 0.697 | 9.343 | |
Trace element solution SL-11 | 1.00 | ml | - | - | |
Bromothymol blue
(0.1% w/v) |
4.00 | ml | 0.004 | 0.006 | |
K2HPO4 | 0.50 | g | 0.498 | 2.856 | |
Na2S2O3 x 5 H2O | 5.00 | g | 4.975 | 20.046 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except hydrogenphosphate and thiosulfate, adjust pH to 6.5, then dispense medium into culture vessels and autoclave. Add hydrogenphosphate and thiosulfate from sterile stock solutions. Adjust pH of complete medium to 6.5 - 7.0. |
864 | HALOTHIOBACILLUS MEDIUM |