MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 873

Download this solution as

CSV JSON PDF

Internal Solution-ID: S1803

Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.33 g 0.329 6.157
KH2PO4 0.33 g 0.329 2.42
KCl 0.33 g 0.329 4.418
NaHCO3 0.80 g 0.798 9.504
Yeast extract
(OXOID) 		0.25 g 0.25 -
Peptone
(BD Bacto) 		0.25 g 0.25 -
Trace element solution SL-10 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
MgCl2 x 6 H2O 0.33 g 0.329 1.62
CaCl2 x 2 H2O 0.22 g 0.22 1.493
Na2CO3 1.50 g 1.497 14.124
D-Glucose 3.00 g 2.994 16.619
Wolin's vitamin solution (10x) 1.00 ml - -
Na2S x 9 H2O 0.50 g 0.499 2.078
Distilled water 1000.00 ml - -
1 Dissolve ingredients, except magnesium chloride, calcium chloride, carbonate, vitamins, glucose and sulfide, then sparge medium for 30 - 45 min with 80% N2 and 20% CO2 gas mixture to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving, add magnesium chloride, calcium chloride, vitamins, glucose and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins should be sterilized by filtration. Adjust pH of the complete medium to 7.0 - 7.3, if necessary.
873 THERMOANAEROVIBRIO MEDIUM