Main sol. 886
Internal Solution-ID: S1834
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.500 | g | 0.5 | 3.674 | |
| NH4Cl | 0.500 | g | 0.5 | 9.348 | |
| NaCl | 0.500 | g | 0.5 | 8.556 | |
| MgCl2 x 6 H2O | 0.600 | g | 0.6 | 2.951 | |
| CaCl2 | 0.100 | g | 0.1 | 0.901 | |
| Na2CO3 | 2.500 | g | 2.5 | 23.588 | |
| NaHCO3 | 2.500 | g | 2.5 | 29.759 | |
| Na-acetate | 1.000 | g | 1 | 12.19 | |
| Yeast extract | 0.500 | g | 0.5 | - | |
| Na2S x 9 H2O | 0.400 | g | 0.4 | 1.665 | |
| Vitamin B12 | 20.000 | µg | 2.0e-5 | 1.5e-5 | |
| Biotin | 20.000 | µg | 2.0e-5 | 8.2e-5 | |
| Trace element solution SL-6 | 1.000 | ml | - | - | |
| Distilled water | 1000.000 | ml | - | - | |
| Resazurin | 0.002 | % | 0.02 | 0.087 | |
| 1 Boil the medium for few minutes. Fill in tubes with rubber stoppers under nitrogen gas. Sterilize at 121°C for 15 min. | |||||
| 2 Prepare NaHCO3, Na2CO3 and sulfide as separate sterile stock solution, Vitamin B12 and Biotin as filter-sterilized stock solution and add to sterile medium after autoclaving. Adjust pH to 9 - 9.5 . | |||||
| 3 To control anoxic conditions, add resazurin to a final concentration of 0.002%. | |||||
Media with this solution
| 886 | HELIORESTIS MEDIUM |