Main sol. 888
Internal Solution-ID: S1837
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
(NH4)2SO4 | 1.0 | g | 1 | 7.568 | |
MgSO4 x 7 H2O | 1.0 | g | 1 | 4.057 | |
CaCl2 x 6 H2O | 30.0 | mg | 0.03 | 0.137 | |
Na2HPO4 | 10.0 | mg | 0.01 | 0.07 | |
Sodium succinate | 1.0 | g | 1 | 6.171 | |
Casamino acids
(DIFCO) |
1.5 | g | 1.5 | - | |
Trace elements solution (Pfennig & Lippert,1966) | 1.0 | ml | - | - | |
Agar
(DIFCO) |
0.5 | g | 0.5 | 1.487 | |
Distilled water | 1000.0 | ml | - | - | |
1 pH 7.5 | |||||
2 Add after autoclaving sterile solutions of: | |||||
Na2S2O3 x 5 H2O
(10% solution) |
1.0 | ml | 0.1 | 0.403 | |
Vitamine mixture | 1.0 | ml | - | - |
888 | AQUASPIRILLUM MEDIUM |