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Main sol. 888

Internal Solution-ID: S1837

Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 1.0 g 1 7.568
MgSO4 x 7 H2O 1.0 g 1 4.057
CaCl2 x 6 H2O 30.0 mg 0.03 0.137
Na2HPO4 10.0 mg 0.01 0.07
Sodium succinate 1.0 g 1 6.171
Casamino acids
(DIFCO)
1.5 g 1.5 -
Trace elements solution (Pfennig & Lippert,1966) 1.0 ml - -
Agar
(DIFCO)
0.5 g 0.5 1.487
Distilled water 1000.0 ml - -
1 pH 7.5
2 Add after autoclaving sterile solutions of:
Na2S2O3 x 5 H2O
(10% solution)
1.0 ml 0.1 0.403
Vitamine mixture 1.0 ml - -
888 AQUASPIRILLUM MEDIUM