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Main sol. 916

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Internal Solution-ID: S1878

Equipment needed: Gassing station Autoclave Hungate tubes Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 912 ml - -
Solution B 50 ml - -
Solution C 25 ml - -
Solution D 10 ml - -
Solution E 1 ml - -
Solution F 10 ml - -
1 Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions B and D are autoclaved under 100% N2 gas atmosphere. Solution C is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solutions E and F are prepared under 100% N2 gas and sterilized by filtration. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. The pH of the complete medium should be 7.0 - 7.2.
2 Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning.
916 DESULFOFABA HANSENII MEDIUM