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916. DESULFOMUSA HANSENII MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 900 ml - -
2 Solution B 1 ml - -
3 Solution C 1 ml - -
4 Solution D 25 ml - -
5 Solution E 10 ml - -
6 Solution F 50 ml - -
7 Solution G 10 ml - -
8 Solution H 10 ml - -
9 , may stimulate growth of some strains 20 mg 0.02 -
Instructions:
  1. Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions B, C, F, G and H are autoclaved separately under 100% N2 gas atmosphere. Solution D is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas and sterilized by filtration. To complete the medium appropriate amounts of solutions B to H are added to the sterile solution A in the sequence as indicated. The pH of the complete medium should be 7.0 - 7.2.
  2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5%(w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 20.00 g 22.222 380.241
2 0.67 g 0.744 9.986
3 0.10 g 0.111 2.077
4 0.01 g 0.011 0.082
5 0.02 g 0.022 0.09
6 1.42 g 1.578 11.108
7
(0.1% w/v)
0.50 ml 5.6e-4 0.002
8 900.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace element solution SL-10 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Selenite-tungstate solution 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.25 g 50 471.752
2 25.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Vitamin solution 10 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 10.60 g 212 1042.78
2 1.52 g 30.4 206.782
3 50.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.52 g 52 261.554
2 10.00 ml 998.207 55408.8
Instructions:
  1. Adjust to pH 2 with HCl.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.96 g 96 999.375
2 10.00 ml 998.207 55408.8
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.1
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.197 55408.2
NaCl 19.861 339.838
MgCl2 x 6 H2O 10.526 51.777
CaCl2 x 2 H2O 1.509 10.267
Na2SO4 1.41 9.927
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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