Main sol. 929
Internal Solution-ID: S1913
Equipment needed: Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 26.40 | g | 26.4 | 451.745 | |
| MgCl2 x 6 H2O | 5.70 | g | 5.7 | 28.037 | |
| MgSO4 x 7 H2O | 6.80 | g | 6.8 | 27.589 | |
| CaCl2 x 2 H2O | 1.50 | g | 1.5 | 10.203 | |
| KCl | 0.66 | g | 0.66 | 8.853 | |
| KBr | 0.09 | g | 0.09 | 0.756 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare the medium anaerobically under an atmosphere of N2:CO2 (8:2 vol:vol) and then add: | |||||
|
NaHCO3
(84 g/l autoclaved under a CO2 atmopshere) |
30.00 | ml/l | 2.52 | 29.998 | |
| KH2PO4 | 0.40 | g/l | 0.4 | 2.939 | |
| NH4Cl | 0.25 | g/l | 0.25 | 4.674 | |
| Na2S2O3 | 0.50 | mM | 0.214 | 0.5 | |
| Trace elements | 1.00 | ml/l | - | - | |
| Selenite/tungsate solution | 1.00 | ml/l | - | - | |
| Vitamin solution | 1.00 | ml/l | - | - | |
| Vitamin B12 solution 0.05 g/L | 1.00 | ml/l | - | - | |
| Vitamin B1 solution 0.1 g/L | 1.00 | ml/l | - | - | |
| Acetate | 5.00 | mM | 0.295 | 5 | |
| FeSO4 | 10.00 | mM | 1.519 | 10 | |
| 2 The pH is adjusted to 6.8 | |||||
| 3 To the medium add 5 mM acetate and 10 mM FeSO4 (from a 1 M stock solution kept under nitrogen). When the iron is added to the medium a white floculant precipitate may appear. In the initial stages of growth the precipitate turns orange/brown as the ferrous ions are oxidised to ferrihydrite. Cultivation may take 2-3 weeks at a distance of 30 cm from a 25 W light bulb. | |||||
Media with this solution
| 929 | RHODOVULUM IODOSUM AND R. ROBIGINOSUM MEDIUM |