1
All solutions are prepared anaerobically under N2/CO2 (80:20) gas and autoclaved. After cooling, the solutions are combined under N2/CO2 (80:20). Add 1 ml/l vitamin solution. pH should be 7.5 without further adjustment.
2
Instead of chlorate, nitrate at a final concentration of 0.85 g/l may be used as electron
acceptor.
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