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DSMZ

DSMZ 944a . MEDIUM MODIFIED FOR DECHLOROMONAS SPP.

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 944a #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 900 ml - -
Solution B 50 ml - -
Solution C 50 ml - -
Vitamin solution 1 ml - -
1 All solutions are prepared anaerobically under N2/CO2 (80:20) gas and autoclaved. After cooling, the solutions are combined under N2/CO2 (80:20). Add 1 ml/l vitamin solution. pH should be 7.5 without further adjustment.
2 Instead of chlorate, nitrate at a final concentration of 0.85 g/l may be used as electron acceptor.

Solution A #

900
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.41 g 0.456 3.348
Na2HPO4 0.53 g 0.589 4.148
NaClO3 1.06 g 1.178 -
Na-acetate x 3 H2O 2.72 g 3.022 -
Yeast extract 0.10 g 0.111 -
Resazurin 0.50 mg 5.6e-4 0.002
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 4.00 ml - -
Distilled water 900.00 ml - -

Solution B #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 0.11 g 2.2 19.823
MgCl2 0.10 g 2 21.006
Distilled water 50.00 ml - -

Solution C #

50
Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4HCO3 0.44 g 8.8 -
NaHCO3 3.73 g 74.6 888.021
Na2SO4 0.20 g 4 28.161
Distilled water 50.00 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite-tungstate solution #

1000
Solution occurs in 179 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Vitamin solution #

1000
Solution occurs in 15 other media (first defined in Medium 461).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 50 mg 0.05 0.037
Pantothenic acid 50 mg 0.05 0.228
Riboflavin 50 mg 0.05 0.133
Pyridoxamine hydrochloride 10 mg 0.01 0.049
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Nicotinic acid 25 mg 0.025 0.203
Nicotine amide 25 mg 0.025 0.205
alpha-lipoic acid 50 mg 0.05 0.242
p-Aminobenzoic acid 50 mg 0.05 0.365
Thiamine-HCl x 2 H2O 50 mg 0.05 0.134
Distilled water 1000 ml - -
1 Stir for some hours, filter sterilize the solution.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 3
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 3.73 44.401
Na-acetate x 3 H2O 2.72 -
NaClO3 1.06 -
Na2HPO4 0.53 3.733
NH4HCO3 0.44 -
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
oxygen anaerobe 2 strains
oxygen aerobe 1 strain
oxygen microaerophile 1 strain
temperature mesophilic 3 strains