Main sol. 985
Internal Solution-ID: S2014
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 18.00 | g | 17.946 | 307.087 | |
| MgCl2 x 6 H2O | 4.00 | g | 3.988 | 19.616 | |
| KCl | 0.34 | g | 0.339 | 4.547 | |
| NH4Cl | 0.25 | g | 0.249 | 4.66 | |
| CaCl2 | 0.11 | g | 0.11 | 0.988 | |
| K2HPO4 | 0.18 | g | 0.179 | 1.03 | |
| Fe(NH4)2(SO4)2 x 7 H2O | 20.00 | mg | 0.02 | 0.049 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Yeast extract | 0.20 | g | 0.199 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 1.50 | g | 1.496 | 14.11 | |
| KNO3 | 1.00 | g | 0.997 | 9.861 | |
| Na-acetate | 1.60 | g | 1.595 | 19.446 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Na2S x 9 H2O | 0.50 | g | 0.499 | 2.076 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, nitrate, acetate, vitamins and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins (sterilized by filtration), nitrate, acetate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. The pH of the complete medium should be 6.5 -7.0. | |||||
Media with this solution
| 985 | DEFERRIBACTER AUTOTROPHICUS MEDIUM |