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Main sol. 1010

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Internal Solution-ID: S2057

Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 26.40 g 26.295 449.946
MgCl2 x 6 H2O 5.70 g 5.677 27.925
MgSO4 x 7 H2O 6.80 g 6.773 27.479
KCl 0.66 g 0.657 8.818
CaCl2 x 2 H2O 1.47 g 1.464 9.959
KBr 0.09 g 0.09 0.753
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
KH2PO4 0.20 g 0.199 1.464
NH4Cl 0.25 g 0.249 4.655
Na2CO3 1.00 g 0.996 9.397
Na-DL-lactate 2.30 g 2.291 20.443
Wolin's vitamin solution (10x) 1.00 ml - -
Seven vitamins solution 1.00 ml - -
Na2S x 9 H2O 0.30 g 0.299 1.244
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except dihydrogenphosphate, ammonium chloride, carbonate, lactate, vitamins and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add dihydrogenphosphate, ammonium chloride, lactate, vitamins and sulfide form sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. The vitamin solutions should be sterilized by filtration. Adjust pH of the complete medium to 7.2 - 7.5, if necessary.
1010 DESULFOPILA CORRODENS MEDIUM