Main sol. 1055
Internal Solution-ID: S2132
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 100.0 | g | 99.701 | 1706.04 | |
| MgSO4 x 7 H2O | 10.0 | g | 9.97 | 40.451 | |
| KCl | 6.0 | g | 5.982 | 80.242 | |
| CaCl2 x 2 H2O | 0.4 | g | 0.399 | 2.713 | |
| NH4Cl | 1.0 | g | 0.997 | 18.639 | |
| KH2PO4 | 0.1 | g | 0.1 | 0.733 | |
| Yeast extract | 0.5 | g | 0.499 | - | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
| Selenite-tungstate solution | 1.0 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 1.5 | g | 1.496 | 14.11 | |
| Na-L-lactate | 2.5 | g | 2.493 | - | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| Na2S x 9 H2O | 0.3 | g | 0.299 | 1.245 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, lactate, vitamins, and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add lactate, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of the complete medium to 7.0 - 7.2, if necessary. | |||||
Media with this solution
| 1055 | DESULFOVERMICULUS MEDIUM |