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Main sol. 1056

Internal Solution-ID: S2133

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	60.0	g	59.821	1023.62
MgSO₄ x 7 H₂O	10.0	g	9.97	40.451
KCl	6.0	g	5.982	80.242
CaCl₂ x 2 H₂O	0.4	g	0.399	2.713
NH₄Cl	1.0	g	0.997	18.639
KH₂PO₄	0.1	g	0.1	0.733
Yeast extract	0.5	g	0.499	-
Trace element solution SL-10	1.0	ml	-	-
Selenite-tungstate solution	1.0	ml	-	-
Sodium resazurin (0.1% w/v)	0.5	ml	5.0e-4	0.002
Na₂CO₃	1.5	g	1.496	14.11
Na-propionate	1.5	g	1.496	15.569
Wolin's vitamin solution (10x)	1.0	ml	-	-
Na₂S x 9 H₂O	0.3	g	0.299	1.245
Distilled water	1000.0	ml	-	-
1 Dissolve ingredients (except carbonate, propionate, vitamins and sulfide) and sparge medium with 80% N₂ and 20% CO₂ gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add propionate, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N₂ gas and carbonate from a sterile anoxic stock solution prepared under 80% N₂ and 20% CO₂ gas mixture. Adjust pH of the complete medium to 7.0 - 7.2, if necessary.
2 Prior to use add sterile 100% H₂ gas to 0.5 bar overpressure.
3 Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N₂ and filter-sterilized) can be used to completely reduce the medium and may stimulate growth at the beginning.

Compound

Amount

Unit

Conc. [g/L]

Conc. [mM]

NaCl

60.0

59.821

1023.62

MgSO₄ x 7 H₂O

10.0

9.97

40.451

KCl

6.0

5.982

80.242

CaCl₂ x 2 H₂O

0.4

0.399

2.713

NH₄Cl

1.0

0.997

18.639

KH₂PO₄

0.1

0.733

Yeast extract

0.5

0.499

Trace element solution SL-10

1.0

Selenite-tungstate solution

1.0

Sodium resazurin
(0.1% w/v)

0.5

5.0e-4

0.002

Na₂CO₃

1.5

1.496

14.11

Na-propionate

1.5

1.496

15.569

Wolin's vitamin solution (10x)

1.0

Na₂S x 9 H₂O

0.3

0.299

1.245

Distilled water

1000.0

1 Dissolve ingredients (except carbonate, propionate, vitamins and sulfide) and sparge medium with 80% N₂ and 20% CO₂ gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add propionate, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N₂ gas and carbonate from a sterile anoxic stock solution prepared under 80% N₂ and 20% CO₂ gas mixture. Adjust pH of the complete medium to 7.0 - 7.2, if necessary.

2 Prior to use add sterile 100% H₂ gas to 0.5 bar overpressure.

3 Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N₂ and filter-sterilized) can be used to completely reduce the medium and may stimulate growth at the beginning.

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Main sol. 1056

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