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Hank's BSS

Internal Solution-ID: S2183

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution 1 25 ml - -
Solution 2 25 ml - -
Water 450 ml - -
1 25 ml solution 1 (4 g NaCl, 0.2 g KCl, 0.05 g MgSO4 x 7 H2O, 0.05 g MgCl2 x 6 H2O dissolved in 25 ml distilled water; then 0.0925 g CaCl2 added and dissolved) and 25 ml of solution 2 (0.075 g Na2HPO4 x 2 H2O, 0.03 g KH2PO4 dissolved in 25 ml distilled water) are autoclaved separately and then combined with 450 ml sterile H2O.
1078 FRIIS MEDIUM