Main sol. 1081
Internal Solution-ID: S2189
This solution is used in 2 media and was first defined in medium 1081.
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| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Glucose | 10.0 | g | 10 | 55.506 | |
| Polypeptone | 5.0 | g | 5 | - | |
| Yeast extract | 5.0 | g | 5 | - | |
| K2HPO4 | 1.0 | g | 1 | 5.741 | |
| MgSO4 x 7 H2O | 0.2 | g | 0.2 | 0.811 | |
| Agar | 15.0 | g | 15 | 44.599 | |
| Distilled water | 900.0 | ml | - | - | |
|
Na2CO3
(10 %) |
100.0 | ml | 10 | 94.35 | |
| 1 After autoclaving, aseptically add 100 ml of a 10% Na2CO3 solution to the medium. Check final pH to be about 10.0. | |||||