Main sol. 1099
Internal Solution-ID: S2215
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 20.0 | g | 19.98 | 341.889 | |
| KH2PO4 | 0.5 | g | 0.5 | 3.671 | |
| NH4Cl | 1.0 | g | 0.999 | 18.676 | |
| Na2SO4 | 1.0 | g | 0.999 | 7.033 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
| MgSO4 x 7 H2O | 2.0 | g | 1.998 | 8.106 | |
| Yeast extract | 0.5 | g | 0.5 | - | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
| Na-L-lactate | 2.5 | g | 2.498 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.844 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except cysteine) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add cysteine form a sterile anoxic stock solution prepared under 100% N2 gas and adjust pH of the complete medium to 6.5, if necessary. | |||||
Media with this solution
| 1099 | DESULFOLUNA MEDIUM |