Main sol. 1126
Internal Solution-ID: S2260
Equipment needed: Anaerobic workbench
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 0.25 | g | 0.25 | 1.435 | |
| KH2PO4 | 1.00 | g | 1 | 7.348 | |
| (NH4)2SO4 | 1.00 | g | 1 | 7.568 | |
| CaCl2 | 0.10 | g | 0.1 | 0.901 | |
| MgSO4 | 0.40 | g | 0.4 | 3.323 | |
| Na-EDTA | 0.01 | g | 0.01 | 0.03 | |
| FeCl3 x 6 H2O | 1.00 | mg | 1.0e-3 | 0.004 | |
| KI | 0.20 | mg | 2.0e-4 | 7.7e-4 | |
| CoCl2 x 6 H2O | 0.20 | mg | 2.0e-4 | 0.002 | |
| MnCl2 x 4 H2O | 0.80 | mg | 8.0e-4 | 0.004 | |
| ZnSO4 | 0.80 | mg | 8.0e-4 | 0.005 | |
| H3BO2 | 0.10 | mg | 1.0e-4 | 0.002 | |
| Na2MoO4 x 2 H2O | 0.10 | mg | 1.0e-4 | 4.1e-4 | |
| CuCl2 | 0.10 | mg | 1.0e-4 | 7.4e-4 | |
| NiCl2 x 6 H2O | 0.20 | mg | 2.0e-4 | 0.002 | |
| Sodium citrate | 2.00 | g | 2 | 7.75 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare the medium anaerobically under nitrogen. Adjust to pH 5.5 - 6.5 with 1M H3PO4. Dispense into serum bottles or Hungate tubes (under a nitrogen atmosphere) that can be sealed with rubber stoppers. | |||||
Media with this solution
| 1126 | TELMATOSPIRILLUM MEDIUM |