Main sol. 1145
Internal Solution-ID: S2296
Equipment needed: Hungate tubes
Anaerobic workbench
Water bath
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Double distilled water
(anaerobic) |
800.00 | ml | - | - | |
| MS Buffer | 200.00 | ml | - | - | |
| Elemental sulphur | 5.00 | g | 5 | - | |
| NaS2O3 x 5 H2O | 2.00 | g | 2 | - | |
| MgSO4 x 7 H2O | 7.00 | g | 7 | 28.4 | |
| KCl | 0.48 | g | 0.48 | 6.439 | |
| CaCl2 x 2 H2O | 0.40 | g | 0.4 | 2.721 | |
| MgCl2 x 6 H2O | 0.80 | g | 0.8 | 3.935 | |
| Solution A | 20.00 | ml | - | - | |
| Solution B | 1.50 | ml | - | - | |
| Solution C - Trace mineral solution (Ferguson and Mah, 1983) | 10.00 | ml | - | - | |
| 1 Each of the solutions (A, B, and C) is prepared with anaerobic double distilled water. | |||||
| 2 The medium is prepared anaerobically under nitrogen gas and dispensed into Hungate tubes or serum bottle sealed with rubber septa. The medium to gas phase ratio should be about 1:10 (v:v). Sterilise the medium by heating at 100° C for 3 hours on three consecutive days (e.g., in a water bath). | |||||
| 3 Add 4% O2 for microaerophilic conditions. | |||||
Media with this solution
| 1145 | MS-MEDIUM (MODIFIED) |