This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Double distilled water
(anaerobic) |
800.00 | ml | - | - | |
MS Buffer | 200.00 | ml | - | - | |
Elemental sulphur | 5.00 | g | 5 | - | |
NaS2O3 x 5 H2O | 2.00 | g | 2 | - | |
MgSO4 x 7 H2O | 7.00 | g | 7 | 28.4 | |
KCl | 0.48 | g | 0.48 | 6.439 | |
CaCl2 x 2 H2O | 0.40 | g | 0.4 | 2.721 | |
MgCl2 x 6 H2O | 0.80 | g | 0.8 | 3.935 | |
Solution A | 20.00 | ml | - | - | |
Solution B | 1.50 | ml | - | - | |
Solution C - Trace mineral solution (Ferguson and Mah, 1983) | 10.00 | ml | - | - | |
1 Each of the solutions (A, B, and C) is prepared with anaerobic double distilled water. | |||||
2 The medium is prepared anaerobically under nitrogen gas and dispensed into Hungate tubes or serum bottle sealed with rubber septa. The medium to gas phase ratio should be about 1:10 (v:v). Sterilise the medium by heating at 100° C for 3 hours on three consecutive days (e.g., in a water bath). | |||||
3 Add 4% O2 for microaerophilic conditions. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaOH | 100 | mM | 4 | 100 | |
1 100 mM NaOH saturated with CO2 |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 100 | g | 100 | 1869.51 | |
MgCl2 x 6 H2O | 100 | g | 100 | 491.877 | |
CaCl2 x 2 H2O | 40 | g | 40 | 272.081 | |
Distilled water | 1000 | ml | - | - | |
1 Adjust to pH 4 with HCl |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
K2HPO4 x 3 H2O | 200 | g | 200 | 1148.26 |
Distilled water | 1000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2-EDTA x 2 H2O | 500 | mg | 0.5 | 1.487 | |
Na2WO4 x 2 H2O | 30 | mg | 0.03 | 0.091 | |
CoCl2 x 6 H2O | 150 | mg | 0.15 | 1.155 | |
CuCl2 x 2 H2O | 20 | mg | 0.02 | 0.117 | |
MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 | |
NiSO4 x 6 H2O | 20 | mg | 0.02 | 0.129 | |
FeSO4 x 7 H2O | 100 | mg | 0.1 | 0.36 | |
H2SeO3 | 10 | mg | 0.01 | 0.078 | |
ZnCl2 | 100 | mg | 0.1 | 0.734 | |
H3BO3 | 10 | mg | 0.01 | 0.162 | |
AlCl3 x 6 H2O | 40 | mg | 0.04 | 0.166 | |
Na2MoO4 x 2 H2O | 10 | mg | 0.01 | 0.041 | |
Distilled water | 1000 | ml | - | - | |
1 Adjust pH to 3.0 with HCl |
Last modified: | 15.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | n.d. |
Equipment needed: | Anaerobic workbench, Hungate tubes, Water bath |
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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
MgSO4 x 7 H2O | 7 | 28.4 |
Elemental sulphur | 5 | - |
NH4Cl | 2 | 37.39 |
MgCl2 x 6 H2O | 2 | 9.838 |
NaS2O3 x 5 H2O | 2 | - |
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