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Main sol. 1146

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Internal Solution-ID: S2301

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KCl 0.50 g 0.5 6.707
MgCl2 x 6 H2O 1.36 g 1.36 6.69
MgSO4 x 7 H2O 7.00 g 7 28.4
Na2S2O3 x 5 H2O 2.00 g 2 8.058
CaCl2 x 2 H2O 0.40 g 0.4 2.721
NH4Cl 0.20 g 0.2 3.739
KH2PO4 0.25 g 0.25 1.837
Trace mineral solution 10.00 ml - -
MES 1.95 g 1.95 9.988
Distilled water 1000.00 ml - -
1 Adjust the pH to 5.5 and gas the media with N2/CO2 (80/20) for 15 min at least. Fill 20 ml medium into 100 ml serum bottles, gas the flask containing the media with H2/CO2 (80/20) and seal with a rubber stopper. Autoclave the medium for 20 min at 121°C. After the inoculation the tubes are pressurized with H2/CO2/O2 (79%/15%/6%,170 kPa).
1146 VENENIVIBRIO STAGNISPUMANTIS MEDIUM